ArticlePloS one2015
Calling Biomarkers in Milk Using a Protein Microarray on Your Smartphone.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- Advancing Point-of-Care Diagnosis: Digitalizing Combinatorial Biomarker Signals for Lupus Nephritis.Biosensors · 2024Article
- Smartphone-Based Quantitative Analysis of Protein Array Signals for Biomarker Detection in Lupus.Chemosensors (Basel, Switzerland) · 2022Article
- Microsphere Peptide-Based Immunoassay for the Detection of Recombinant Bovine Somatotropin in Injection Preparations.Biosensors · 2022Article
- The Promise and Challenges of Determining Recombinant Bovine Growth Hormone in Milk.Foods (Basel, Switzerland) · 2022Review
- Review
- Nano-Biosensing Platforms for Detection of Cow's Milk Allergens: An Overview.Sensors (Basel, Switzerland) · 2019Review
- The Efficiency of Color Space Channels to Quantify Color and Color Intensity Change in Liquids, pH Strips, and Lateral Flow Assays with Smartphones.Sensors (Basel, Switzerland) · 2019Article
- Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study.Scientific reports · 2019Article
- Emerging Point-of-care Technologies for Food Safety Analysis.Sensors (Basel, Switzerland) · 2019Review
- Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity.ACS omega · 2019Article
- Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes.Biosensors · 2018Article
- "The Smartphone's Guide to the Galaxy": In Situ Analysis in Space.Biosensors · 2018Review
- Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays.Analytical and bioanalytical chemistry · 2018Review
- New portable smartphone-based PDMS microfluidic kit for the simultaneous colorimetric detection of arsenic and mercury.RSC advances · 2018Article
- Dual-Color Bioluminescent Sensor Proteins for Therapeutic Drug Monitoring of Antitumor Antibodies.Analytical chemistry · 2018Article
- A smart device application for the automated determination ofEngineering in life sciences · 2017Article
- High-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader.Scientific reports · 2016Article
- An Efficient Power Harvesting Mobile Phone-Based Electrochemical Biosensor for Point-of-Care Health Monitoring.Sensors and actuators. B, Chemical · 2016Article
- 3-D Printed Adjustable Microelectrode Arrays for Electrochemical Sensing and Biosensing.Sensors and actuators. B, Chemical · 2016Article
- Multimodal Imaging and Lighting Bias Correction for Improved μPAD-based Water Quality Monitoring via Smartphones.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we present the concept of a protein microarray-based fluorescence immunoassay for multiple biomarker detection in milk extracts by an ordinary smartphone. A multiplex immunoassay was designed on a microarray chip, having built-in positive and negative quality controls. After the immunoassay procedure, the 48 microspots were labelled with Quantum Dots (QD) depending on the protein biomarker levels in the sample. QD-fluorescence was subsequently detected by the smartphone camera under UV light excitation from LEDs embedded in a simple 3D-printed opto-mechanical smartphone attachment. The somewhat aberrant images obtained under such conditions, were corrected by newly developed Android-based software on the same smartphone, and protein biomarker profiles were calculated. The indirect detection of recombinant bovine somatotropin (rbST) in milk extracts based on altered biomarker profile of anti-rbST antibodies was selected as a real-life challenge. RbST-treated and untreated cows clearly showed reproducible treatment-dependent biomarker profiles in milk, in excellent agreement with results from a flow cytometer reference method. In a pilot experiment, anti-rbST antibody detection was multiplexed with the detection of another rbST-dependent biomarker, insulin-like growth factor 1 (IGF-1). Milk extract IGF-1 levels were found to be increased after rbST treatment and correlated with the results obtained from the reference method. These data clearly demonstrate the potential of the portable protein microarray concept towards simultaneous detection of multiple biomarkers. We envisage broad application of this 'protein microarray on a smartphone'-concept for on-site testing, e.g., in food safety, environment and health monitoring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.