ArticleAnalytical chemistry2015
Generation of multiple reporter ions from a single isobaric reagent increases multiplexing capacity for quantitative proteomics.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Advancements in Single-Cell Proteomics and Mass Spectrometry-Based Techniques for Unmasking Cellular Diversity in Triple Negative Breast Cancer.Proteomics. Clinical applications · 2025Review
- Chemical isotope labeling for quantitative proteomics.Mass spectrometry reviews · 2023Review
- Single-cell proteomics enabled by next-generation sequencing or mass spectrometry.Nature methods · 2023Review
- Isobaric labeling: Expanding the breadth, accuracy, depth, and diversity of sample multiplexing.Proteomics · 2022Article
- Review
- Review of the Real and Sometimes Hidden Costs in Proteomics Experimental Workflows.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Strategies for mass spectrometry-based phosphoproteomics using isobaric tagging.Expert review of proteomics · 2021Review
- iDRiP for the systematic discovery of proteins bound directly to noncoding RNA.Nature protocols · 2021Article
- Defining the carrier proteome limit for single-cell proteomics.Nature methods · 2021Article
- Mass Spectrometry Advances and Perspectives for the Characterization of Emerging Adoptive Cell Therapies.Molecules (Basel, Switzerland) · 2020Review
- Evaluating False Transfer Rates from the Match-between-Runs Algorithm with a Two-Proteome Model.Journal of proteome research · 2019Article
- A Review on Quantitative Multiplexed Proteomics.Chembiochem : a European journal of chemical biology · 2019Review
- Multiplexed Relative Quantitation with Isobaric Tagging Mass Spectrometry Reveals Class I Major Histocompatibility Complex Ligand Dynamics in Response to Doxorubicin.Analytical chemistry · 2019Article
- TKO6: A Peptide Standard To Assess Interference for Unit-Resolved Isobaric Labeling Platforms.Journal of proteome research · 2019Article
- Sample Multiplexing Strategies in Quantitative Proteomics.Analytical chemistry · 2019Review
- Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster.Analytical chemistry · 2018Article
- Using hyperLOPIT to perform high-resolution mapping of the spatial proteome.Nature protocols · 2017Article
- Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT).Journal of visualized experiments : JoVE · 2017Article
- A Strategy to Combine Sample Multiplexing with Targeted Proteomics Assays for High-Throughput Protein Signature Characterization.Molecular cell · 2017Article
- A Biologist's Field Guide to Multiplexed Quantitative Proteomics.Molecular & cellular proteomics : MCP · 2016Review
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Authors and funding
8 authors.
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Abstract
Isobaric labeling strategies for mass spectrometry-based proteomics enable multiplexed simultaneous quantification of samples and therefore substantially increase the sample throughput in proteomics. However, despite these benefits, current limits to multiplexing capacity are prohibitive for large sample sizes and impose limitations on experimental design. Here, we introduce a novel mechanism for increasing the multiplexing density of isobaric reagents. We present Combinatorial Isobaric Mass Tags (CMTs), an isobaric labeling architecture with the unique ability to generate multiple series of reporter ions simultaneously. We demonstrate that utilization of multiple reporter ion series improves multiplexing capacity of CMT with respect to a commercially available isobaric labeling reagent with preserved quantitative accuracy and depth of coverage in complex mixtures. We provide a blueprint for the realization of 16-plex reagents with 1 Da spacing between reporter ions and up to 28-plex at 6 mDa spacing using only 5 heavy isotopes per reagent. We anticipate that this improvement in multiplexing capacity will further advance the application of quantitative proteomics, particularly in high-throughput screening assays.
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