ArticlePloS one2015
Spinning-Spot Shadowless TIRF Microscopy.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Multimodal holotomography for volumetric monitoring of host-pathogen interactions.Biomedical engineering online · 2026Article
- Multiscale imaging of polarity in bovine oviductal organoids.Frontiers in cell and developmental biology · 2026Article
- GRIN2B disease-associated mutations disrupt the function of BK channels and NMDA receptor signaling nanodomains.The Journal of general physiology · 2025Article
- C. elegans epicuticlins define specific compartments in the apical extracellular matrix and function in wound repair.Development (Cambridge, England) · 2024Article
- Photonic lantern TIRF microscopy for highly efficient, uniform, artifact-free imaging.Optics express · 2024Article
- Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.Nature communications · 2024Article
- Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis.Nature communications · 2024Article
- Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.Biomedical optics express · 2024Article
- Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis.Nature microbiology · 2024Article
- DeepBacs for multi-task bacterial image analysis using open-source deep learning approaches.Communications biology · 2022Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- High-resolution imaging of bacterial spatial organization with vertical cell imaging by nanostructured immobilization (VerCINI).Nature protocols · 2022Review
- FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division.Nature communications · 2021Article
- Single-shot, shadowless total internal reflection fluorescence microscopy via annular fiber bundle.Optics letters · 2020Article
- Cell-wide mapping of Orai1 channel activity reveals functional heterogeneity in STIM1-Orai1 puncta.The Journal of general physiology · 2020Article
- Stability and nuclear localization of yeast telomerase depend on protein components of RNase P/MRP.Nature communications · 2020Article
- Metasurface-based total internal reflection microscopy.Biomedical optics express · 2020Article
- VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy.Optics express · 2019Article
- Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation.Journal of imaging · 2019Article
- Myosin-II mediated traction forces evoke localized Piezo1-dependent CaCommunications biology · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing near-membrane cellular structures and processes, including imaging of local Ca2+ transients with single-channel resolution. TIRF is most commonly implemented in epi-fluorescence mode, whereby laser excitation light is introduced at a spot near the periphery of the back focal plane of a high numerical aperture objective lens. However, this approach results in an irregular illumination field, owing to interference fringes and scattering and shadowing by cellular structures. We describe a simple system to circumvent these limitations, utilizing a pair of galvanometer-driven mirrors to rapidly spin the laser spot in a circle at the back focal plane of the objective lens, so that irregularities average out during each camera exposure to produce an effectively uniform field. Computer control of the mirrors enables precise scanning at 200 Hz (5ms camera exposure times) or faster, and the scan radius can be altered on a frame-by-frame basis to achieve near-simultaneous imaging in TIRF, widefield and 'skimming plane' imaging modes. We demonstrate the utility of the system for dynamic recording of local inositol trisphosphate-mediated Ca2+ signals and for imaging the redistribution of STIM and Orai proteins during store-operated Ca2+ entry. We further anticipate that it will be readily applicable for numerous other near-membrane studies, especially those involving fast dynamic processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.