ArticleMarine drugs2015
Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum.
Article in Marine drugs, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Review
- Metabolic engineering for improved heterologous pinene production in the chloroplast of Phaeodactylum tricornutum.Microbial cell factories · 2026Article
- Installing a Ketocarotenoid Branch inMarine drugs · 2025Article
- Elucidating the Molecular Mechanisms of Pulsed Light-Induced Lycopene Accumulation in Tomatoes Through Integrated Multi-Omics Analysis.International journal of molecular sciences · 2025Article
- The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives.Plant communications · 2025Review
- Metabolic engineering and cultivation strategies for efficient production of fucoxanthin and related carotenoids.Applied microbiology and biotechnology · 2025Review
- A key gene, violaxanthin de-epoxidase-like 1, enhances fucoxanthin accumulation in Phaeodactylum tricornutum.Biotechnology for biofuels and bioproducts · 2024Article
- Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation.Marine drugs · 2024Article
- Identification of Potential Factors for the Promotion of Fucoxanthin Synthesis by Methyl Jasmonic Acid Treatment ofMarine drugs · 2023Article
- Roles of Two Phytoene Synthases and Orange Protein in Carotenoid Metabolism of the β-Carotene-AccumulatingMicrobiology spectrum · 2023Article
- Microalgae-Derived Pigments for the Food Industry.Marine drugs · 2023Review
- Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production.Current issues in molecular biology · 2022Review
- Emerging Trends in Genetic Engineering of Microalgae for Commercial Applications.Marine drugs · 2022Review
- Optimal Nitrate Supplementation inFoods (Basel, Switzerland) · 2022Article
- Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects.Frontiers in bioengineering and biotechnology · 2022Review
- Recent Progress on Systems and Synthetic Biology of Diatoms for Improving Algal Productivity.Frontiers in bioengineering and biotechnology · 2022Review
- Enhancement of violaxanthin accumulation inFrontiers in microbiology · 2022Article
- Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in LiveMarine drugs · 2021Article
- Hyperaccumulation of fucoxanthin by enhancing methylerythritol phosphate pathway in Phaeodactylum tricornutum.Applied microbiology and biotechnology · 2021Article
- Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development.Foods (Basel, Switzerland) · 2021Review
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Authors and funding
10 authors.
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Abstract
Carotenoids exert beneficial effects on human health through their excellent antioxidant activity. To increase carotenoid productivity in the marine Pennales Phaeodactylum tricornutum, we genetically engineered the phytoene synthase gene (psy) to improve expression because RNA-sequencing analysis has suggested that the expression level of psy is lower than other enzyme-encoding genes that are involved in the carotenoid biosynthetic pathway. We isolated psy from P. tricornutum, and this gene was fused with the enhanced green fluorescent protein gene to detect psy expression. After transformation using the microparticle bombardment technique, we obtained several P. tricornutum transformants and confirmed psy expression in their plastids. We investigated the amounts of PSY mRNA and carotenoids, such as fucoxanthin and β-carotene, at different growth phases. The introduction of psy increased the fucoxanthin content of a transformants by approximately 1.45-fold relative to the levels in the wild-type diatom. However, some transformants failed to show a significant increase in the carotenoid content relative to that of the wild-type diatom. We also found that the amount of PSY mRNA at log phase might contribute to the increase in carotenoids in the transformants at stationary phase.
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