Evidence map›Paper›PMID 26307707›Full record

ArticleEuropean journal of mass spectrometry (Chichester, England)2015

Differentiating chondroitin sulfate glycosaminoglycans using collision-induced dissociation; uronic acid cross-ring diagnostic fragments in a single stage of tandem mass spectrometry.

Muchena J Kailemia, Anish B Patel, Dane T Johnson, Lingyun Li, Robert J Linhardt, I Jonathan Amster

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of mass spectrometry (Chichester, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
  3. A Scoring Algorithm for the Automated Analysis of Glycosaminoglycan MS/MS Data.Journal of the American Society for Mass Spectrometry · 2019
    Article
  4. Article
  5. Structural elucidation of fucosylated chondroitin sulfates from sea cucumber using FTICR-MS/MS.European journal of mass spectrometry (Chichester, England) · 2018
    Article
  6. Sequencing the Dermatan Sulfate Chain of Decorin.Journal of the American Chemical Society · 2017
    Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Muchena J KailemiaDepartment of Chemistry, University of Georgia, Athens, GA 30602, USA. jkmuchena@ucdavis.edu.
Anish B PatelDe partment of Chemistry, University of Georgia, Athens, GA 30602, USA. abpatel@uga.edu.
Dane T JohnsonDepartment of Chemistry, University of Georgia, Athens, GA 30602, USA. d04@uga.edu.
Lingyun LiDepartment of Chemistry and Chemical Biology, Chemical and Biological Engineering, and Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. lil12@rpi.edu.
Robert J LinhardtDepartment of Chemistry and Chemical Biology, Chemical and Biological Engineering, and Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. linhar@rpi.edu.
I Jonathan AmsterDepartment of Chemistry, University of Georgia, Athens, GA 30602, USA. jamster@uga.edu.
University of Georgia · USRensselaer Polytechnic Institute · US

Funding

TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY W. · 2012 to 2019
$14.5M
Purchase of a Thermo Fisher Scientific LTQ Orbitrap XL Mass SpectrometerS10RR028859 · NCRR · UNIVERSITY OF GEORGIA · PI AMSTER, I JONATHAN · 2011 to 2011
$832k
NCRR NIH HHS S10 RR028859NIGMS NIH HHS P41 GM103390
6 · The paper itself

Abstract

The stereochemistry of the hexuronic acid residues of the structure of glycosaminoglycans (GAGs) is a key feature that affects their interactions with proteins and other biological functions. Electron based tandem mass spectrometry methods, in particular electron detachment dissociation (EDD), have been able to distinguish glucuronic acid (GlcA) from iduronic acid (IdoA) residues in some heparan sulfate tetrasaccharides by producing epimer-specific fragments. Similarly, the relative abundance of glycosidic fragment ions produced by collision-induced dissociation (CID) or EDD has been shown to correlate with the type of hexuronic acid present in chondroitin sulfate GAGs. The present work examines the effect of charge state and degree of sodium cationization on the CID fragmentation products that can be used to distinguish GlcA and IdoA containing chondroitin sulfate A and dermatan sulfate chains. The cross-ring fragments (2,4)A(n) and (0,2)X(n) formed within the hexuronic acid residues are highly preferential for chains containing GlcA, distinguishing it from IdoA. The diagnostic capability of the fragments requires the selection of a molecular ion and fragment ions with specific ionization characteristics, namely charge state and number of ionizable protons. The ions with the appropriate characteristics display diagnostic properties for all the chondroitin sulfate and dermatan sulfate chains (degree of polymerization of 4-10) studied.

Indexed as

Chondroitin SulfatesChromatography, High Pressure LiquidReproducibility of ResultsSensitivity and SpecificitySpectroscopy, Fourier Transform InfraredUronic Acidschondroitin sulfate glycosaminoglycanChondroitin SulfatesUronic Acids

Identifiers

PMID26307707
PMCPMC4552082
OpenAlexW1903885269

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.