ArticleBlood2015
Free hemoglobin increases von Willebrand factor-mediated platelet adhesion in vitro: implications for circulatory devices.
Article in Blood, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 93 citations in OpenAlex.
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- Haemolytic injury and cardiovascular disease.Nature reviews. Cardiology · 2026Review
- The Role of Platelet-Derived Microparticles in Platelet Adhesion and Aggregation to Immobilized Extracellular Matrix Proteins Under Flow.Cellular and molecular bioengineering · 2026Article
- Free Fetal Haemoglobin in Severe Early-Onset Fetal Growth Restriction: A Prospective Multi-Centre Study.BJOG : an international journal of obstetrics and gynaecology · 2026Article
- Cell-free hemoglobin is associated with microcirculatory perfusion disturbances and acute kidney injury in rats on extracorporeal membrane oxygenation.BMC anesthesiology · 2025Article
- Enhancing prediction and stratifying risk: machine learning and bayesian-learning models for catheter-related thrombosis in chemotherapy patients.BMC cancer · 2025Article
- Recent Advances and Future Directions in Extracorporeal Carbon Dioxide Removal.Journal of clinical medicine · 2024Review
- Thrombosis in Paroxysmal Nocturnal Hemoglobinuria (PNH): From Pathogenesis to Treatment.International journal of molecular sciences · 2024Review
- Hemocompatibility and biophysical interface of left ventricular assist devices and total artificial hearts.Blood · 2024Review
- Extracorporeal Membrane Oxygenation (ECMO)-Associated Coagulopathy in Adults.Diagnostics (Basel, Switzerland) · 2023Review
- Hemostatic complications and systemic heparinization in pediatric post-cardiotomy veno-arterial extracorporeal membrane oxygenation failed to wean from cardiopulmonary bypass.Translational pediatrics · 2022Article
- Thrombus formation during ECMO: Insights from a detailed histological analysis of thrombus composition.Journal of thrombosis and haemostasis : JTH · 2022Article
- Hyper-reactive platelets and type 2 diabetes.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2022Article
- Development of Novel Amphotericin B-Immobilized Nitric Oxide-Releasing Platform for the Prevention of Broad-Spectrum Infections and Thrombosis.ACS applied materials & interfaces · 2021Article
- Thrombotic Complications in Patients with Immune-Mediated Hemolysis.Journal of clinical medicine · 2021Review
- Selective potassium uptake via biocompatible zeolite-polymer hybrid microbeads as promising binders for hyperkalemia.Bioactive materials · 2021Article
- Hemolysis at low blood flow rates: in-vitro and in-silico evaluation of a centrifugal blood pump.Journal of translational medicine · 2021Article
- COVID-19 and Extracorporeal Membrane Oxygenation.Advances in experimental medicine and biology · 2021Review
- Neurological Monitoring and Complications of Pediatric Extracorporeal Membrane Oxygenation Support.Pediatric neurology · 2020Review
- Changes in Red Blood Cell Properties and Platelet Function during Extracorporeal Membrane Oxygenation.Journal of clinical medicine · 2020Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Intravascular hemolysis occurs in patients on extracorporeal membrane oxygenation. High levels of free acellular adult hemoglobin (free HbA) are associated with clotting in this mechanical device that can result in thrombotic complications. Adsorption of fibrinogen onto the surface of biomaterial correlates with platelet adhesion, which is mediated by von Willebrand factor (VWF). Because free Hb interacts with VWF, we studied the effect of hemoglobin (Hb) on platelet adhesion to fibrin(ogen) under conditions of different hydrodynamic forces. This effect was investigated using purified human HbA and fibrinogen, extracellular matrix, collagen, or purified plasma VWF as surface-coated substrates to examine flow-dependent platelet adhesion. Antibodies and VWF-deficient plasma were also used. Free Hb (≥50 mg/dL) effectively augmented platelet adhesion, and microthrombi formation on fibrin(ogen), extracellular matrix, and collagen at high shear stress. The effect of free Hb was effectively blocked by anti-glycoprotein Ibα (GPIbα) antibodies or depletion of VWF. Unexpectedly, free Hb also promoted firm platelet adhesion and stable microthrombi on VWF. Lastly, we determined that Hb interacts directly with the A1 domain. This study is the first to demonstrate that extracellular Hb directly affects the GPIbα-VWF interaction in thrombosis, and describes another mechanism by which hemolysis is connected to thrombotic events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.