ArticleThe Journal of biological chemistry2015
Characterization of the Role of β-Carotene 9,10-Dioxygenase in Macular Pigment Metabolism.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 71 citations in OpenAlex.
- Regulation of apocarotenoids for quality improvement and biofortification of horticultural crops.Journal of advanced research · 2026Review
- Carotenoid bioavailability in humans reflects the mammalian nocturnal bottleneck: a position paper.Journal of clinical biochemistry and nutrition · 2026Article
- Retinoid dynamics in vision: from visual cycle biology to retina disease treatments.Pharmacology & therapeutics · 2025Review
- Characterization of astaxanthin isomers in different types of salmon filets and human plasma after salmon consumption.Food chemistry · 2025Article
- Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells.Antioxidants (Basel, Switzerland) · 2025Article
- Unveiling BCO2 function in macular pigment metabolism: Mitochondrial processing and expression in the primate retina.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2025Article
- Imaging macular carotenoids and their related proteins in the human retina with confocal resonance Raman and fluorescence microscopy.Experimental eye research · 2024Article
- Article
- miR-330 targeting BCO2 is involved in carotenoid metabolism to regulate skin pigmentation in rainbow trout (Oncorhynchus mykiss).BMC genomics · 2023Article
- Aster la vista: Unraveling the biochemical basis of carotenoid homeostasis in the human retina.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Aster proteins mediate carotenoid transport in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fenretinide inhibits vitamin A formation from β-carotene and regulates carotenoid levels in mice.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2022Article
- Carotenoid modifying enzymes in metazoans.Methods in enzymology · 2022Article
- Comparison of Antioxidant Properties of Dehydrolutein with Lutein and Zeaxanthin, and their Effects on Cultured Retinal Pigment Epithelial Cells.Antioxidants (Basel, Switzerland) · 2021Article
- The Structural and Biochemical Basis of Apocarotenoid Processing by β-Carotene Oxygenase-2.ACS chemical biology · 2021Article
- Xanthophylls Modulate Palmitoylation of Mammalian β-Carotene Oxygenase 2.Antioxidants (Basel, Switzerland) · 2021Article
- β-carotene oxygenase 2 deficiency-triggered mitochondrial oxidative stress promotes low-grade inflammation and metabolic dysfunction.Free radical biology & medicine · 2021Article
- Molecular components affecting ocular carotenoid and retinoid homeostasis.Progress in retinal and eye research · 2021Review
- The human mitochondrial enzyme BCO2 exhibits catalytic activity toward carotenoids and apocarotenoids.The Journal of biological chemistry · 2020Article
- The macular carotenoids: A biochemical overview.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A family of enzymes collectively referred to as carotenoid cleavage oxygenases is responsible for oxidative conversion of carotenoids into apocarotenoids, including retinoids (vitamin A and its derivatives). A member of this family, the β-carotene 9,10-dioxygenase (BCO2), converts xanthophylls to rosafluene and ionones. Animals deficient in BCO2 highlight the critical role of the enzyme in carotenoid clearance as accumulation of these compounds occur in tissues. Inactivation of the enzyme by a four-amino acid-long insertion has recently been proposed to underlie xanthophyll concentration in the macula of the primate retina. Here, we focused on comparing the properties of primate and murine BCO2s. We demonstrate that the enzymes display a conserved structural fold and subcellular localization. Low temperature expression and detergent choice significantly affected binding and turnover rates of the recombinant enzymes with various xanthophyll substrates, including the unique macula pigment meso-zeaxanthin. Mice with genetically disrupted carotenoid cleavage oxygenases displayed adipose tissue rather than eye-specific accumulation of supplemented carotenoids. Studies in a human hepatic cell line revealed that BCO2 is expressed as an oxidative stress-induced gene. Our studies provide evidence that the enzymatic function of BCO2 is conserved in primates and link regulation of BCO2 gene expression with oxidative stress that can be caused by excessive carotenoid supplementation.
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