Evidence map›Paper›PMID 26306320›Full record

ArticleData in brief2015

Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi.

Maggy T Sikulu, James Monkman, Keyur A Dave, Marcus L Hastie, Patricia E Dale, Roger L Kitching, Gerry F Killeen, Brian H Kay, Jeffry J Gorman, Leon E Hugo

Open access · goldAbstract read
In one paragraph

Article in Data in brief, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. DIGE Analysis Software and Protein Identification Approaches.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

Maggy T SikuluMosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
James MonkmanMosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Keyur A DaveProtein Discovery Centre, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Marcus L HastieProtein Discovery Centre, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Patricia E DaleEnvironmental Futures Research Institute and Griffith School of Environment, Griffith University, Brisbane, Queensland, Australia.
Roger L KitchingEnvironmental Futures Research Institute and Griffith School of Environment, Griffith University, Brisbane, Queensland, Australia.
Gerry F KilleenEnvironmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania ; Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
Brian H KayMosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Jeffry J GormanProtein Discovery Centre, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Leon E HugoMosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
QIMR Berghofer Medical Research Institute · AUGriffith University · AULiverpool School of Tropical Medicine · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

Identifiers

PMID26306320
PMCPMC4534588
OpenAlexW915062568

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.