Evidence map›Paper›PMID 26306199›Full record

ArticleIUCrJ2015

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells.

Jose A Rodriguez, Rui Xu, Chien-Chun Chen, Zhifeng Huang, Huaidong Jiang, Allan L Chen, Kevin S Raines, Alan Pryor, Daewoong Nam, Lutz Wiegart and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in IUCrJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

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  9. Stochastic chromatin packing of 3D mitotic chromosomes revealed by coherent X-rays.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jose A RodriguezBiological Chemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA.
Rui XuDepartment of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA.
Chien-Chun ChenDepartment of Physics, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Zhifeng HuangCarl ZEISS X-ray Microscopy Inc., Pleasanton, CA 94588, USA.
Huaidong JiangState Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, People's Republic of China.
Allan L ChenDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Kevin S RainesDepartment of Applied Physics, Stanford University, Stanford, CA 94305, USA.
Alan PryorDepartment of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA.
Daewoong NamDepartment of Physics, Pohang University of Science and Technology, Pohang 790-784, South Korea.
Lutz WiegartNSLS-II Photon Sciences Division, Brookhaven National Laboratory, Upton, NY 11973, USA.
Changyong SongDepartment of Physics, Pohang University of Science and Technology, Pohang 790-784, South Korea.
Anders MadsenEuropean X-ray Free Electron Laser, Albert-Einstein-Ring 19, Hamburg 22761, Germany.
Yuriy ChushkinESRF, The European Synchrotron, 71 Avenue des Martyrs, Grenoble, France.
Federico ZontoneESRF, The European Synchrotron, 71 Avenue des Martyrs, Grenoble, France.
Peter J BradleyDepartment of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Jianwei MiaoDepartment of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA.

Funding

The Role of Toxoplasma Rhoptries in Host Cell InfectionR01AI064616 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRADLEY, PETER JOHN · 2006 to 2016
$3.5M
Three-Dimensional Imaging of Whole Cells by Using X-ray Diffraction MicroscopyR01GM081409 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MIAO, JIANWEI · 2009 to 2010
$470k
NIAID NIH HHS R01 AI064616NIGMS NIH HHS R01 GM081409
6 · The paper itself

Abstract

A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.

Indexed as

coherent diffractioncoherent diffractive imagingcryo-CDINeospora caninumthree-dimensional cellular structurethree-dimensional imagingX-ray imaging

Identifiers

PMID26306199
PMCPMC4547825

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.