ArticleVirulence2015
Adaptation of a Gaussia princeps Luciferase reporter system in Candida albicans for in vivo detection in the Galleria mellonella infection model.
Article in Virulence, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Bridging the gap in AMR research: validation of a standardizedFrontiers in pharmacology · 2026Article
- Fungal infections in focus: accelerating non-invasive imaging from preclinical insights to clinical breakthroughs.Npj imaging · 2025Review
- Sensitive bioluminescence imaging of cryptococcosis inVirulence · 2024Article
- AlternativeFrontiers in cellular and infection microbiology · 2024Review
- Powerful and Real-Time Quantification of Antifungal Efficacy against Triazole-Resistant and -Susceptible Aspergillus fumigatus Infections in Galleria mellonella by Longitudinal Bioluminescence Imaging.Microbiology spectrum · 2023Article
- Review
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Aequorin as a Useful Calcium-Sensing Reporter inJournal of fungi (Basel, Switzerland) · 2021Article
- A Novel Immunocompetent Mouse Model for Testing Antifungal Drugs Against InvasiveJournal of fungi (Basel, Switzerland) · 2020Article
- Optimization and Characterization of aFrontiers in microbiology · 2019Article
- Article
- Methodologies forMicrobial cell (Graz, Austria) · 2018Review
- Establishment and Validation of Galleria mellonella as a Novel Model Organism To Study Mycobacterium abscessus Infection, Pathogenesis, and Treatment.Antimicrobial agents and chemotherapy · 2018Article
- The Rim Pathway Mediates Antifungal Tolerance in Candida albicans through Newly Identified Rim101 Transcriptional Targets, Including Hsp90 and Ipt1.Antimicrobial agents and chemotherapy · 2018Article
- In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging.Virulence · 2018Review
- Identification and Mode of Action of a Plant Natural Product Targeting Human Fungal Pathogens.Antimicrobial agents and chemotherapy · 2017Article
- The Candida albicans Pho4 Transcription Factor Mediates Susceptibility to Stress and Influences Fitness in a Mouse Commensalism Model.Frontiers in microbiology · 2016Article
- Lightning up the worm: How to probe fungal virulence in an alternative mini-host by bioluminescence.Virulence · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the past 10 years, mini-host models and in particular the greater wax moth Galleria mellonella have tended to become a surrogate for murine models of fungal infection mainly due to cost, ethical constraints and ease of use. Thus, methods to better assess the fungal pathogenesis in G. mellonella need to be developed. In this study, we implemented the detection of Candida albicans cells expressing the Gaussia princeps luciferase in its cell wall in infected larvae of G. mellonella. We demonstrated that detection and quantification of luminescence in the pulp of infected larvae is a reliable method to perform drug efficacy and C. albicans virulence assays as compared to fungal burden assay. Since the linearity of the bioluminescent signal, as compared to the CFU counts, has a correlation of R(2) = 0.62 and that this method is twice faster and less labor intensive than classical fungal burden assays, it could be applied to large scale studies. We next visualized and followed C. albicans infection in living G. mellonella larvae using a non-toxic and water-soluble coelenterazine formulation and a CCD camera that is commonly used for chemoluminescence signal detection. This work allowed us to follow for the first time C. albicans course of infection in G. mellonella during 4 days.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.