ArticleThe ISME journal2016
Metabolic footprint of epiphytic bacteria on Arabidopsis thaliana leaves.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 146 citations in OpenAlex.
- Adaxial-abaxial leaf surface asymmetry is a key ecological driver of the phyllosphere microbiome.Journal of experimental botany · 2026Article
- Whole-genome sequence of Rahnella aceris HPDA25 isolated from mycorrhizosphere of Gastrodia elata.BMC genomic data · 2026Article
- Metabolic feedbacks drive population dynamics and can lead to oscillations among leaf bacteria.Nature communications · 2026Article
- Phyllosphere microbiome: Exploring the unexplored frontiers for precision agricultural and environmental sustainability.World journal of microbiology & biotechnology · 2026Review
- Multimodal Analysis of Phytoalexin Synthesis in Arabidopsis by Mass Spectrometry Imaging and Fluorescent Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Arabidopsis root defense barriers support beneficial interactions with rhizobacterium Pseudomonas simiae WCS417.The New phytologist · 2025Article
- Species-level bacterial community shift in rice growth stages.Plant biotechnology (Tokyo, Japan) · 2025Article
- The effects of small geographical resolution and age on the phyllosphere microbial diversity ofMicrobiology spectrum · 2025Article
- Role of metabolism, resistance, and/or antagonism as drivers of endomicrobiomes assemblage in Origanum heracleoticum L.Communications biology · 2025Article
- Differential Responses of Methylobacterium and Sphingomonas Species to Multispecies Interactions in the Phyllosphere.Environmental microbiology · 2025Article
- Community standards and future opportunities for synthetic communities in plant-microbiota research.Nature microbiology · 2024Review
- The Exometabolome ofMetabolites · 2024Article
- Multiomics Reveals the Key Microorganisms and Metabolites in the Resistance to Root Rot Disease ofGenes · 2023Article
- Relationships between Phyllosphere Bacterial Communities and Leaf Functional Traits in a Temperate Forest.Plants (Basel, Switzerland) · 2023Article
- Phyllosphere Microbiome in Plant Health and Disease.Plants (Basel, Switzerland) · 2023Review
- Characterization of Hsp17, a Novel Small Heat Shock Protein, in Sphingomonas melonis TY under Heat Stress.Microbiology spectrum · 2023Article
- The Arabidopsis holobiont: a (re)source of insights to understand the amazing world of plant-microbe interactions.Environmental microbiome · 2023Review
- Revealing ecotype influences onFrontiers in microbiology · 2023Article
- Metabolic adaptation to vitamin auxotrophy by leaf-associated bacteria.The ISME journal · 2022Article
- Pseudomonas syringae pv. tomato infection of tomato plants is mediated by GABA and l-Pro chemoperception.Molecular plant pathology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phyllosphere, which is defined as the parts of terrestrial plants above the ground, is a large habitat for different microorganisms that show a high extent of adaption to their environment. A number of hypotheses were generated by culture-independent functional genomics studies to explain the competitiveness of specialized bacteria in the phyllosphere. In contrast, in situ data at the metabolome level as a function of bacterial colonization are lacking. Here, we aimed to obtain new insights into the metabolic interplay between host and epiphytes upon colonization of Arabidopsis thaliana leaves in a controlled laboratory setting using environmental metabolomics approaches. Quantitative nuclear magnetic resonance (NMR) and imaging high-resolution mass spectrometry (IMS) methods were used to identify Arabidopsis leaf surface compounds and their possible involvement in the epiphytic lifestyle by relative changes in compound pools. The dominant carbohydrates on the leaf surfaces were sucrose, fructose and glucose. These sugars were significantly and specifically altered after epiphytic leaf colonization by the organoheterotroph Sphingomonas melonis or the phytopathogen Pseudomonas syringae pv. tomato, but only to a minor extent by the methylotroph Methylobacterium extorquens. In addition to carbohydrates, IMS revealed surprising alterations in arginine metabolism and phytoalexin biosynthesis that were dependent on the presence of bacteria, which might reflect the consequences of bacterial activity and the recognition of not only pathogens but also commensals by the plant. These results highlight the power of environmental metabolomics to aid in elucidating the molecular basis underlying plant-epiphyte interactions in situ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.