ArticlePlant physiology2015
The Calcium Ion Is a Second Messenger in the Nitrate Signaling Pathway of Arabidopsis.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers, 1 of them a synthesis that pooled it.
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Who cites it
104 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Primary nitrate responses mediated by calcium signalling and diverse protein phosphorylation.Journal of experimental botany · 2020Pooled it
- Beyond membrane transport: Transporters as dynamic signal integrators for plant signaling and adaptation.Plant communications · 2026Review
- Root Transporters Shape Rhizosphere Microbiomes to Enhance Nitrogen Acquisition Efficiency in Plants.Microorganisms · 2026Review
- HHO5 orchestrates dose-dependent feedback regulation of organic versus inorganic nitrogen signaling in Arabidopsis.The Plant cell · 2026Article
- A plasma membrane Ca2+-dependent protein kinase PtCDPK2 promotes phosphorus starvation resilience in Phaeodactylum tricornutum.Journal of experimental botany · 2026Article
- The NRT1.1-NLP7 Nexus: An Integrative Signaling Nexus from Nitrate Sensing to Systemic Adaptation and Structure-Guided Engineering.Plants (Basel, Switzerland) · 2026Review
- Nitrogen-Controlled Host Gatekeeping: Regulatory Chokepoints Across Four Windows for Diazotroph Access.International journal of molecular sciences · 2026Article
- Progress and new questions in understanding the mechanisms controlling plant nitrogen responses.Plant biotechnology (Tokyo, Japan) · 2026Article
- Cell-type-specific immune programs orchestrate spatial defense in the Arabidopsis leaf epidermis.Nature communications · 2026Article
- Physiological and Molecular Mechanisms of Nitrogen Regulation on Grain Quality in Cereal Crops at Later Stages.International journal of molecular sciences · 2026Review
- Functional characterization of NRT1/PTR FAMILY transporters: looking for a needle in a haystack.The New phytologist · 2026Review
- QuinoaFrontiers in plant science · 2026Article
- NRT1.1 as central signaling hub orchestrating plant growth and stress trade-offs.Frontiers in plant science · 2026Review
- Genome-wide association study, linkage mapping and transcriptomic analysis reveal candidate genes associated with nitrogen use efficiency in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- An evolutionary conserved CBL1-CIPK6 complex of oil persimmon involved in responses to ABA, salt and drought stress.BMC plant biology · 2025Article
- Meltome Atlas of Arabidopsis thaliana proteome: a melting temperature-based identification of heat and cold resistant proteins using in-silico approach.BMC genomics · 2025Article
- OsNRT1.1B-OsCNGC14/16-CaAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Nitrogen-Driven Orchestration of Lateral Root Development: Molecular Mechanisms and Systemic Integration.Biology · 2025Review
- Chromatin accessibility dynamics and transcriptional regulatory networks underlying the primary nitrogen response in rice roots.Plant communications · 2025Article
- Abiotic Stress-Induced Chloroplast and Cytosolic CaPlant, cell & environment · 2025Article
44 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
Understanding how plants sense and respond to changes in nitrogen availability is the first step toward developing strategies for biotechnological applications, such as improvement of nitrogen use efficiency. However, components involved in nitrogen signaling pathways remain poorly characterized. Calcium is a second messenger in signal transduction pathways in plants, and it has been indirectly implicated in nitrate responses. Using aequorin reporter plants, we show that nitrate treatments transiently increase cytoplasmic Ca(2+) concentration. We found that nitrate also induces cytoplasmic concentration of inositol 1,4,5-trisphosphate. Increases in inositol 1,4,5-trisphosphate and cytoplasmic Ca(2+) levels in response to nitrate treatments were blocked by U73122, a pharmacological inhibitor of phospholipase C, but not by the nonfunctional phospholipase C inhibitor analog U73343. In addition, increase in cytoplasmic Ca(2+) levels in response to nitrate treatments was abolished in mutants of the nitrate transceptor NITRATE TRANSPORTER1.1/Arabidopsis (Arabidopsis thaliana) NITRATE TRANSPORTER1 PEPTIDE TRANSPORTER FAMILY6.3. Gene expression of nitrate-responsive genes was severely affected by pretreatments with Ca(2+) channel blockers or phospholipase C inhibitors. These results indicate that Ca(2+) acts as a second messenger in the nitrate signaling pathway of Arabidopsis. Our results suggest a model where NRT1.1/AtNPF6.3 and a phospholipase C activity mediate the increase of Ca(2+) in response to nitrate required for changes in expression of prototypical nitrate-responsive genes.
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