ArticlePlant physiology2015
Phosphorylation and Dephosphorylation of the Presequence of Precursor MULTIPLE ORGANELLAR RNA EDITING FACTOR3 during Import into Mitochondria from Arabidopsis.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Evolutionary Refinement of Mitochondrial and Plastid Targeting Sequences Coincides with the Late Diversification of Land Plants.Molecular biology and evolution · 2025Article
- Molecular basis for maternal inheritance of human mitochondrial DNA.Nature genetics · 2023Article
- The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles.Plants (Basel, Switzerland) · 2022Article
- Overlapping Functions of the Paralogous Proteins AtPAP2 and AtPAP9 inInternational journal of molecular sciences · 2021Article
- A Balance between the Activities of Chloroplasts and Mitochondria Is Crucial for Optimal Plant Growth.Antioxidants (Basel, Switzerland) · 2021Article
- OsPPR939, a nad5 splicing factor, is essential for plant growth and pollen development in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Evidence for thermosensitivity of the cotton (Gossypium hirsutum L.) immature fiber (im) mutant via hypersensitive stomatal activity.PloS one · 2021Article
- Modulating the activities of chloroplasts and mitochondria promotes adenosine triphosphate production and plant growth.Quantitative plant biology · 2021Article
- Differential RNA Editing and Intron Splicing in Soybean Mitochondria during Nodulation.International journal of molecular sciences · 2020Article
- Triple-localized WHIRLY2 Influences Leaf Senescence and Silique Development via Carbon Allocation.Plant physiology · 2020Article
- Article
- Multiple Kinases Can Phosphorylate the N-Terminal Sequences of Mitochondrial Proteins inFrontiers in plant science · 2018Article
- Multiple PPR protein interactions are involved in the RNA editing system inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transgenic Arabidopsis thaliana containing increased levels of ATP and sucrose is more susceptible to Pseudomonas syringae.PloS one · 2017Article
- Article
- AtPAP2 modulates the import of the small subunit of Rubisco into chloroplasts.Plant signaling & behavior · 2016Article
- Characterization of purple acid phosphatases involved in extracellular dNTP utilization in Stylosanthes.Journal of experimental botany · 2016Article
- Transcriptomic, proteomic and metabolic changes in Arabidopsis thaliana leaves after the onset of illumination.BMC plant biology · 2016Article
- Impacts of high ATP supply from chloroplasts and mitochondria on the leaf metabolism of Arabidopsis thaliana.Frontiers in plant science · 2015Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleus-encoded mitochondria-targeted proteins, multiple organellar RNA editing factors (MORF3, MORF5, and MORF6), interact with Arabidopsis (Arabidopsis thaliana) PURPLE ACID PHOSPHATASE2 (AtPAP2) located on the chloroplast and mitochondrial outer membranes in a presequence-dependent manner. Phosphorylation of the presequence of the precursor MORF3 (pMORF3) by endogenous kinases in wheat germ translation lysate, leaf extracts, or STY kinases, but not in rabbit reticulocyte translation lysate, resulted in the inhibition of protein import into mitochondria. This inhibition of import could be overcome by altering threonine/serine residues to alanine on the presequence, thus preventing phosphorylation. Phosphorylated pMORF3, but not the phosphorylation-deficient pMORF3, can form a complex with 14-3-3 proteins and HEAT SHOCK PROTEIN70. The phosphorylation-deficient mutant of pMORF3 also displayed faster rates of import when translated in wheat germ lysates. Mitochondria isolated from plants with altered amounts of AtPAP2 displayed altered protein import kinetics. The import rate of pMORF3 synthesized in wheat germ translation lysate into pap2 mitochondria was slower than that into wild-type mitochondria, and this rate disparity was not seen for pMORF3 synthesized in rabbit reticulocyte translation lysate, the latter translation lysate largely deficient in kinase activity. Taken together, these results support a role for the phosphorylation and dephosphorylation of pMORF3 during the import into plant mitochondria. These results suggest that kinases, possibly STY kinases, and AtPAP2 are involved in the import of protein into both mitochondria and chloroplasts and provide a mechanism by which the import of proteins into both organelles may be coordinated.
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