ArticleThe Journal of cell biology2015
An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- ER shaping proteins guide spindle elongation and division during rapid cleavage mitoses.bioRxiv : the preprint server for biology · 2026Article
- The Drosophila nucleoporin ELYS is required for parental chromosome arrangement at fertilization.G3 (Bethesda, Md.) · 2025Article
- Cell state-specific cytoplasmic density controls spindle architecture and scaling.Nature cell biology · 2025Article
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Exclusion of the endoplasmic reticulum from themicroPublication biology · 2025Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofLife science alliance · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofbioRxiv : the preprint server for biology · 2024Article
- Endoplasmic reticulum membranes are continuously required to maintain mitotic spindle size and forces.Life science alliance · 2023Article
- Nuclear tension controls mitotic entry by regulating cyclin B1 nuclear translocation.The Journal of cell biology · 2022Article
- Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo.Scientific reports · 2022Article
- Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes.The Journal of cell biology · 2022Article
- Contribution of cytoplasm viscoelastic properties to mitotic spindle positioning.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.Developmental cell · 2021Article
- Role of spatial patterns and kinetochore architecture in spindle morphogenesis.Seminars in cell & developmental biology · 2021Review
- Nuclear envelope remodelling during mitosis.Current opinion in cell biology · 2021Review
- Review
- C. elegans pronuclei fuse after fertilization through a novel membrane structure.The Journal of cell biology · 2020Article
- Spatio-temporal expression of ANK2 promotes cytokinesis in oocytes.Scientific reports · 2019Article
- REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis.Molecular biology of the cell · 2019Article
- Increased Expression of Maturation Promoting Factor Components Speeds Up Meiosis in Oocytes from Aged Females.International journal of molecular sciences · 2018Article
Corrections and comments
- Commented on by
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitotic spindle is a microtubular assembly required for chromosome segregation during mitosis. Additionally, a spindle matrix has long been proposed to assist this process, but its nature has remained elusive. By combining live-cell imaging with laser microsurgery, fluorescence recovery after photobleaching, and fluorescence correlation spectroscopy in Drosophila melanogaster S2 cells, we uncovered a microtubule-independent mechanism that underlies the accumulation of molecules in the spindle region. This mechanism relies on a membranous system surrounding the mitotic spindle that defines an organelle-exclusion zone that is conserved in human cells. Supported by mathematical modeling, we demonstrate that organelle exclusion by a membrane system causes spatio-temporal differences in molecular crowding states that are sufficient to drive accumulation of mitotic regulators, such as Mad2 and Megator/Tpr, as well as soluble tubulin, in the spindle region. This membranous "spindle envelope" confined spindle assembly, and its mechanical disruption compromised faithful chromosome segregation. Thus, cytoplasmic compartmentalization persists during early mitosis to promote spindle assembly and function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.