Evidence map›Paper›PMID 26304668›Full record

ArticleCirculation2015

Microfluidic Single-Cell Analysis of Transplanted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes After Acute Myocardial Infarction.

Sang-Ging Ong, Bruno C Huber, Won Hee Lee, Kazuki Kodo, Antje D Ebert, Yu Ma, Patricia K Nguyen, Sebastian Diecke, Wen-Yi Chen, Joseph C Wu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed, 2 pooled it
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 2 syntheses or guidelines pooled it, 86 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Refining the Guinea Pig (Cavia porcellus) Cryo-Injury Model for Cardiac Regeneration and Functional Characterization.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025
    Article
  4. Review
  5. Pluripotent stem cell-based approaches for heart repair and the potential of genetic modifications.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Review
  6. Article
  7. Review
  8. Review
  9. Single-cell Technology in Stem Cell Research.Current stem cell research & therapy · 2025
    Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
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  20. Review

3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 2 countries.

Sang-Ging Ong *Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Bruno C Huber *Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Won Hee LeeStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Kazuki KodoStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Antje D EbertStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Yu MaStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Patricia K NguyenStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Sebastian DieckeStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Wen-Yi ChenStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Joseph C WuStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA.
Norwegian Womens Public Health Association · NO

Funding

Induced pluripotent stem cells in the understanding and treatment of heart diseaseU01HL099776 · NHLBI · STANFORD UNIVERSITY · PI LONGAKER, MICHAEL T, WU, JOSEPH C. · 2009 to 2015
$8.2M
Human Induced Pluripotent Stem Cells for Cardiovascular Disease ModelingR01HL113006 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2012 to 2025
$6.8M
NHLBI NIH HHS R01 HL113006NHLBI NIH HHS R01HL113006NHLBI NIH HHS U01 HL09776NHLBI NIH HHS U01 HL099776
6 · The paper itself

Abstract

backgroundHuman induced pluripotent stem cells (iPSCs) are attractive candidates for therapeutic use, with the potential to replace deficient cells and to improve functional recovery in injury or disease settings. Here, we test the hypothesis that human iPSC-derived cardiomyocytes (iPSC-CMs) can secrete cytokines as a molecular basis to attenuate adverse cardiac remodeling after myocardial infarction. METHODS AND

resultsHuman iPSCs were generated from skin fibroblasts and differentiated in vitro with a small molecule-based protocol. Troponin(+) iPSC-CMs were confirmed by immunohistochemistry, quantitative polymerase chain reaction, fluorescence-activated cell sorting, and electrophysiological measurements. Afterward, 2×10(6) iPSC-CMs derived from a cell line transduced with a vector expressing firefly luciferase and green fluorescent protein were transplanted into adult NOD/SCID mice with acute left anterior descending artery ligation. Control animals received PBS injection. Bioluminescence imaging showed limited engraftment on transplantation into ischemic myocardium. However, magnetic resonance imaging of animals transplanted with iPSC-CMs showed significant functional improvement and attenuated cardiac remodeling compared with PBS-treated control animals. To understand the underlying molecular mechanism, microfluidic single-cell profiling of harvested iPSC-CMs, laser capture microdissection of host myocardium, and in vitro ischemia stimulation were used to demonstrate that the iPSC-CMs could release significant levels of proangiogenic and antiapoptotic factors in the ischemic microenvironment.

conclusionsTransplantation of human iPSC-CMs into an acute mouse myocardial infarction model can improve left ventricular function and attenuate cardiac remodeling. Because of limited engraftment, most of the effects are possibly explained by paracrine activity of these cells.

Indexed as

Stem Cell TransplantationAnimalsCell LineFemaleHumansInduced Pluripotent Stem CellsMiceMice, Inbred NODMice, SCIDMicrofluidicsMyocardial InfarctionMyocytes, CardiacRandom AllocationSingle-Cell Analysiscell transplantationmolecular imagingmyocardial infarctionmyocytes, cardiacparacrine communicationstem cells

Identifiers

PMID26304668
PMCPMC4557214
OpenAlexW2152597627

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.