ArticleMolecular and cellular biology2015
Functional Requirements for Fab-7 Boundary Activity in the Bithorax Complex.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed.
- Differential chromatin looping regulated by two GA-binding transcription factors creates an X-specific chromatin environment for dosage compensation.bioRxiv : the preprint server for biology · 2026Article
- The Late Boundary Complex (LBC) Selectively Binds the Fab-7/Fab-8 Boundaries, but not Fab-6 or Mcp, in the Abd-B Regulatory Region of Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2026Article
- 3D chromatin structures precede genome activation in Drosophila embryogenesis.Cell genomics · 2025Article
- GAGA zinc finger transcription factor searches chromatin by 1D-3D facilitated diffusion.Nature structural & molecular biology · 2025Article
- Initiators counteract Polycomb repression and stimulate long-range contacts between enhancers and theOpen biology · 2025Article
- The bxd PRE from Bithorax Complex of Drosophila melanogaster Has Weak Insulator Activity.Doklady. Biochemistry and biophysics · 2025Article
- New Drosophila promoter-associated architectural protein Mzfp1 interacts with CP190 and is required for housekeeping gene expression and insulator activity.Nucleic acids research · 2024Article
- A bioinformatics screen reveals hox and chromatin remodeling factors at the Drosophila histone locus.BMC genomic data · 2023Article
- Localization of the Drosophila pioneer factor GAF to subnuclear foci is driven by DNA binding and required to silence satellite repeat expression.Developmental cell · 2023Article
- lncRNA read-through regulates the BX-C insulatoreLife · 2023Article
- Boundary bypass activity in theOpen biology · 2023Article
- Transcriptional Readthrough Interrupts Boundary Function in Drosophila.International journal of molecular sciences · 2023Article
- Boundary Bypass Activity in thebioRxiv : the preprint server for biology · 2023Article
- Transcriptional read through interrupts boundary function in Drosophila.bioRxiv : the preprint server for biology · 2023Article
- Mechanisms of Interaction between Enhancers and Promoters in ThreeInternational journal of molecular sciences · 2023Review
- CRISPR/Cas9 and FLP-FRT mediated regulatory dissection of the BX-C of Drosophila melanogaster.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Article
- A bioinformatics screen reveals Hox and chromatin remodeling factors at thebioRxiv : the preprint server for biology · 2023Article
- Kinetic principles underlying pioneer function of GAGA transcription factor in live cells.Nature structural & molecular biology · 2022Article
- Dimerization Activity of a Disordered N-Terminal Domain fromInternational journal of molecular sciences · 2022Article
- Fragments of the Fab-3 and Fab-4 Boundaries of the Drosophila melanogaster Bithorax Complex That Include CTCF Sites Are not Effective Insulators.Doklady. Biochemistry and biophysics · 2022Article
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6 authors.
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Abstract
Chromatin boundaries are architectural elements that determine the three-dimensional folding of the chromatin fiber and organize the chromosome into independent units of genetic activity. The Fab-7 boundary from the Drosophila bithorax complex (BX-C) is required for the parasegment-specific expression of the Abd-B gene. We have used a replacement strategy to identify sequences that are necessary and sufficient for Fab-7 boundary function in the BX-C. Fab-7 boundary activity is known to depend on factors that are stage specific, and we describe a novel ∼700-kDa complex, the late boundary complex (LBC), that binds to Fab-7 sequences that have insulator functions in late embryos and adults. We show that the LBC is enriched in nuclear extracts from late, but not early, embryos and that it contains three insulator proteins, GAF, Mod(mdg4), and E(y)2. Its DNA binding properties are unusual in that it requires a minimal sequence of >65 bp; however, other than a GAGA motif, the three Fab-7 LBC recognition elements display few sequence similarities. Finally, we show that mutations which abrogate LBC binding in vitro inactivate the Fab-7 boundary in the BX-C.
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