ArticleJournal of cell science2015
Cytoophidium assembly reflects upregulation of IMPDH activity.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 86 citations in OpenAlex.
- Dynamic control of nucleotide metabolism in physiology and disease.Nature cell biology · 2026Review
- The Impact of Induction of Insulin Secretion in MIN6 Cells on the Extent of Cytoophidia Formation and Their Structural Forms.Iranian journal of biotechnology · 2026Article
- Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.Advances in experimental medicine and biology · 2026Review
- CTPS2 regulates CTP synthetase activity by interacting with CTPS1.Life science alliance · 2025Article
- Y12C mutation disrupts IMPDH cytoophidia and alters cancer metabolism.The FEBS journal · 2025Article
- Depletion of nuclear cytoophidia in Alzheimer's disease.Free neuropathology · 2025Article
- The IMPDH cytoophidium couples metabolism and fetal development in mice.Cellular and molecular life sciences : CMLS · 2024Article
- Light-sensitive phosphorylation regulates retinal IMPDH1 activity and filament assembly.The Journal of cell biology · 2024Article
- Cytoophidia: a conserved yet promising mode of enzyme regulation in nucleotide metabolism.Molecular biology reports · 2024Review
- Agglomeration: when folded proteins clump together.Biophysical reviews · 2023Review
- Article
- The transcriptional state and chromatin landscape of cichlid jaw shape variation across species and environments.Molecular ecology · 2023Article
- CTP synthase: the hissing of the cellular serpent.Histochemistry and cell biology · 2022Review
- Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.International journal of molecular sciences · 2022Article
- The gateway to guanine nucleotides: Allosteric regulation of IMP dehydrogenases.Protein science : a publication of the Protein Society · 2022Review
- Molecular crowding facilitates bundling of IMPDH polymers and cytoophidium formation.Cellular and molecular life sciences : CMLS · 2022Article
- Diversity of mechanisms to control bacterial GTP homeostasis by the mutually exclusive binding of adenine and guanine nucleotides to IMP dehydrogenase.Protein science : a publication of the Protein Society · 2022Article
- IMPDH dysregulation in disease: a mini review.Biochemical Society transactions · 2022Review
- Nuclear IMPDH Filaments in Human Gliomas.Journal of neuropathology and experimental neurology · 2021Article
- ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Cytidine triphosphate synthase (CTPS) and inosine monophosphate dehydrogenase (IMPDH) (both of which have two isoforms) can form fiber-like subcellular structures termed 'cytoophidia' under certain circumstances in mammalian cells. Although it has been shown that filamentation of CTPS downregulates its activity by disturbing conformational changes, the activity of IMPDH within cytoophidia is still unclear. Most previous IMPDH cytoophidium studies were performed under conditions involving inhibitors that impair GTP synthesis. Here, we show that IMPDH forms cytoophidia without inhibition of GTP synthesis. First, we find that an elevated intracellular CTP concentration or treatment with 3'-deazauridine, a CTPS inhibitor, promotes IMPDH cytoophidium formation and increases the intracellular GTP pool size. Moreover, restriction of cell growth triggers the disassembly of IMPDH cytoophidia, implying that their presence is correlated with active cell metabolism. Finally, we show that the presence of IMPDH cytoophidia in mouse pancreatic islet cells might correlate with nutrient uptake in the animal. Collectively, our findings reveal that formation of IMPDH cytoophidia reflects upregulation of purine nucleotide synthesis, suggesting that the IMPDH cytoophidium plays a role distinct from that of the CTPS cytoophidium in controlling intracellular nucleotide homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.