ArticleScientific reports2015
Mass Spectrometry-based PhyloProteomics (MSPP): A novel microbial typing Method.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Proteotyping ofMicroorganisms · 2023Article
- Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity ofDiagnostics (Basel, Switzerland) · 2021Article
- PhyloQuant approach provides insights into Trypanosoma cruzi evolution using a systems-wide mass spectrometry-based quantitative protein profile.Communications biology · 2021Article
- Evaluation of Microsatellite Typing, ITS Sequencing, AFLP Fingerprinting, MALDI-TOF MS, and Fourier-Transform Infrared Spectroscopy Analysis ofJournal of fungi (Basel, Switzerland) · 2020Article
- Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex.Molecules (Basel, Switzerland) · 2020Article
- Genetic diversity and population structure of Tenacibaculum maritimum, a serious bacterial pathogen of marine fish: from genome comparisons to high throughput MALDI-TOF typing.Veterinary research · 2020Article
- Rapid and Culture Free Identification ofFrontiers in microbiology · 2020Article
- Pathogen proteotyping: A rapidly developing application of mass spectrometry to address clinical concerns.Clinical mass spectrometry (Del Mar, Calif.) · 2019Review
- Differentiation ofEuropean journal of microbiology & immunology · 2019Article
- Proteotyping as alternate typing method to differentiate Campylobacter coli clades.Scientific reports · 2019Article
- Proteotyping ofFrontiers in microbiology · 2019Article
- Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates.BMC genomics · 2018Article
- Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP).Journal of visualized experiments : JoVE · 2016Article
- Association ofEuropean journal of microbiology & immunology · 2016Article
- A Designed Experiments Approach to Optimizing MALDI-TOF MS Spectrum Processing Parameters Enhances Detection of Antibiotic Resistance in Campylobacter jejuni.Frontiers in microbiology · 2016Article
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5 authors.
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Abstract
MALDI-TOF-MS of microorganisms, which identifies microbes based on masses of high abundant low molecular weight proteins, is rapidly advancing to become another standard method in clinical routine laboratory diagnostics. Allelic isoforms of these proteins result in varying masses of detectable biomarker ions. These variations give rise to a novel typing method for microorganisms named mass spectrometry-based phyloproteomics (MSPP). The base of MSPP is an amino acid sequence list of allelic isoforms caused by non-synonymous mutations in biomarker genes, which were detectable as mass shifts in an overlay of calibrated MALDI-TOF spectra. Thus, for each isolate a combination of amino acid sequences can be deduced from the scheme of recordable biomarker masses. Performing comparably to laborious multilocus and whole genome sequence typing (wgMLST)-approaches it is feasible to build phyloproteomic dendrograms using hierarchical cluster analysis. MSPP bears a high potential especially for identification of chromosomal localised virulence or antimicrobial resistance factors associated with evolutionary relatedness. In this study the principle of MSPP-typing was demonstrated on a Campylobacter jejuni ssp. jejuni isolate collection and MSPP was compared to MLST.
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