Evidence map›Paper›PMID 26302345›Full record

ArticleZhongguo fei ai za zhi = Chinese journal of lung cancer2015

[Effect of Long Non-coding RNA SPRY4-IT1 on Invasion and Migration of A549 Cells].

Song Chai, Wenting An, Lili Guo, Le Jiang, Zhisong Gao, Shujun Li

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Zhongguo fei ai za zhi = Chinese journal of lung cancer, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Song ChaiCancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Wenting AnCancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Lili GuoCancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Le JiangCancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Zhisong GaoCancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Shujun LiCancer Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Hebei Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectiveThe abnormal expression of human long chain non encoding RNA gene is related to many kinds of tumors. The aim of this study is to investigate the expression of long non-coding RNA maternally expressed gene 3 (SPRY4-IT1) in lung cancer (A549) cells, and to observe the effect of SPRY4-IT1 on the invasion and migration of A549 cells.

methodsThe levels of SPRY4-IT1 in A549 was detected by Real-time PCR. The effects of SPRY4-IT1 on the invasion and migration of A549 cell were analyzed by MTT and Transwell assay. The expression of matrix metalloproteinase (MMP) family proteins was determined by Western blot.

resultsThe invasion and migration of A549 cells were increased after SPRY4-IT1 over-expression. The cell spaces were narrower after SPRY4-IT1 over expression in the wound healing assay. Transwell assays showed that the numbers of transmembrane A549 cells were significantly higher in SPRY4-IT1 over expression group than that in control group (P<0.05). Meanwhile, over expression of SPRY4-IT1 reduced the expression of MMP-2 and MMP-9.

conclusionsOver expression of SPRY4-IT1 enhanced the invasion and migration of A549 cells. MMP-2 and MMP-9 might play an important role in this regulation.
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Indexed as

Cell Line, TumorCell MovementCell ProliferationHumansLung NeoplasmsMatrix Metalloproteinase 2Matrix Metalloproteinase 9RNA, Long Noncodinglong noncoding RNA SPRY4-IT1, humanMatrix Metalloproteinase 2Matrix Metalloproteinase 9RNA, Long Noncoding

Identifiers

PMID26302345
PMCPMC6000226
OpenAlexW1904765960

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.