ArticleJournal of proteome research2015
Proteomic Evidence for Components of Spider Silk Synthesis from Black Widow Silk Glands and Fibers.
Article in Journal of proteome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- Transition From Land to Sea: Comparative Genomics Illuminates the Adaptive Evolution of the Intertidal Spider.Molecular ecology resources · 2026Article
- A newly evolved small secretory peptide enhances mechanical properties of spider silk.Nature communications · 2025Article
- Distinct Dimerization Mechanisms in Silkworm and Spider Silk Proteins Revealed by Mass Photometry.The journal of physical chemistry letters · 2025Article
- Backyard Proteomics: A Case Study with the Black Widow Spider.Journal of proteome research · 2025Article
- Identification and Seasonal Abundance of Web- and Air-Borne Sex Pheromone Components of Western Black Widow Spiders, Latrodectus hesperus.Journal of chemical ecology · 2025Article
- Strategies for Making High-Performance Artificial Spider Silk Fibers.Advanced functional materials · 2024Review
- Origin, structure, and composition of the spider major ampullate silk fiber revealed by genomics, proteomics, and single-cell and spatial transcriptomics.Science advances · 2024Article
- Quantitative Shotgun Proteomic Analysis of Bacteria after Overexpression of Recombinant Spider Miniature Spidroin, MaSp1.International journal of molecular sciences · 2024Article
- Widow spiders alter web architecture and attractiveness in response to same-sex competition for prey and mates, and predation risk.Communications biology · 2023Article
- A molecular atlas reveals the tri-sectional spinning mechanism of spider dragline silk.Nature communications · 2023Article
- Molecular mechanisms of the high performance of spider silks revealed through multi-omics analysis.Biophysics and physicobiology · 2023Article
- Relating spidroin motif prevalence and periodicity to the mechanical properties of major ampullate spider silks.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Origin, structure and functional transition of sex pheromone components in a false widow spider.Communications biology · 2022Article
- Complexity of Spider Dragline Silk.Biomacromolecules · 2022Review
- Shotgun and TMT-Labeled Proteomic Analysis of the Ovarian Proteins of an Insect Vector, Aedes aegypti (Diptera: Culicidae).Journal of insect science (Online) · 2022Article
- Anti-Coagulant and Antimicrobial Recombinant Heparin-Binding Major Ampullate Spidroin 2 (MaSp2) Silk Protein.Bioengineering (Basel, Switzerland) · 2022Article
- Darwin's bark spider shares a spidroin repertoire withOpen biology · 2021Article
- Egg Case Protein 3: A Constituent of Black Widow Spider Tubuliform Silk.Molecules (Basel, Switzerland) · 2021Article
- Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Shifts in morphology, gene expression, and selection underlie web loss in Hawaiian Tetragnatha spiders.BMC ecology and evolution · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous analyses of tissue-specific RNA-seq libraries identified 647 predicted genes that were differentially expressed in silk glands of the Western black widow, Latrodectus hesperus. Only ∼5% of these silk-gland specific transcripts (SSTs) encode spidroins; although the remaining predicted genes presumably encode other proteins associated with silk production, this is mostly unverified. Here, we used proteomic analysis of multiple silk glands and dragline silk fiber to investigate the translation of the differentially expressed genes. We find 48 proteins encoded by the differentially expressed transcripts in L. hesperus major ampullate, minor ampullate, and tubuliform silk glands and detect 17 SST encoded proteins in major ampullate silk fibers. The observed proteins include known silk-related proteins, but most are uncharacterized, with no annotation. These unannotated proteins likely include novel silk-associated proteins. Major and minor ampullate glands have the highest overlap of identified proteins, consistent with their shared, distinctive ampullate shape and the overlapping functions of major and minor ampullate silks. Our study substantiates and prioritizes predictions from differential expression analysis of spider silk gland transcriptomes.
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