ArticleACS nano2015
Dynamics and Energy Contributions for Transport of Unfolded Pertactin through a Protein Nanopore.
Article in ACS nano, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- Coil-Globule versus Field-Driven Coil-Blob Transitions of PEG under Nanopore Confinement.ACS measurement science au · 2026Article
- Nanopore sensing of protein and peptide conformation for point-of-care applications.Nature communications · 2025Review
- Controlled Translocation of Proteins through a Biological Nanopore for Single-Protein Fingerprint Identification.Nano letters · 2024Article
- Blobs form during the single-file transport of proteins across nanopores.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification and Detection of a Peptide Biomarker and Its Enantiomer by Nanopore.ACS central science · 2024Article
- Nanopore Discrimination of Coagulation Biomarker Derivatives and Characterization of a Post-Translational Modification.ACS central science · 2023Article
- Biological nanopores for single-molecule sensing.iScience · 2022Review
- Herding cats: Label-based approaches in protein translocation through nanopore sensors for single-molecule protein sequence analysis.iScience · 2021Review
- Detection of single peptide with only one amino acid modification via electronic fingerprinting using reengineered durable channel of Phi29 DNA packaging motor.Biomaterials · 2021Article
- Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.RSC advances · 2021Review
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Nanopore detection of metal ions: Current status and future directions.Small methods · 2020Article
- Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors.Nature communications · 2018Article
- Nano-channel of viral DNA packaging motor as single pore to differentiate peptides with single amino acid difference.Biomaterials · 2018Article
- From current trace to the understanding of confined media.The European physical journal. E, Soft matter · 2018Article
- The Utility of Nanopore Technology for Protein and Peptide Sensing.Proteomics · 2018Review
- Article
- The aerolysin nanopore: from peptidomic to genomic applications.Nanoscale · 2018Review
- Dynamics of a polyelectrolyte through aerolysin channel as a function of applied voltage and concentrationThe European physical journal. E, Soft matter · 2018Article
- Chasing a Protein's Tail: Detection of Polypeptide Translocation through Nanopores.Biophysical journal · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
To evaluate the physical parameters governing translocation of an unfolded protein across a lipid bilayer, we studied protein transport through aerolysin, a passive protein channel, at the single-molecule level. The protein model used was the passenger domain of pertactin, an autotransporter virulence protein. Transport of pertactin through the aerolysin nanopore was detected as transient partial current blockades as the unfolded protein partially occluded the aerolysin channel. We compared the dynamics of entry and transport for unfolded pertactin and a covalent end-to-end dimer of the same protein. For both the monomer and the dimer, the event frequency of current blockades increased exponentially with the applied voltage, while the duration of each event decreased exponentially as a function of the electrical potential. The blockade time was twice as long for the dimer as for the monomer. The calculated activation free energy includes a main enthalpic component that we attribute to electrostatic interactions between pertactin and the aerolysin nanopore (despite the low Debye length), plus an entropic component due to confinement of the unfolded chain within the narrow pore. Comparing our experimental results to previous studies and theory suggests that unfolded proteins cross the membrane by passing through the nanopore in a somewhat compact conformation according to the "blob" model of Daoud and de Gennes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.