ArticleFrontiers in plant science2015
Functional characterization and reconstitution of ABA signaling components using transient gene expression in rice protoplasts.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- An Engineered Abscisic Acid Receptor Enhances ABA Signaling and Improves Abiotic Stress Tolerance in Rice.Plant, cell & environment · 2026Article
- Protoplast-Based Functional Genomics and Genome Editing: Progress, Challenges and Applications.Plant, cell & environment · 2026Review
- Article
- OsSDG715, a Histone H3K9 Methyltransferase, Integrates Auxin and Cytokinin Signaling to Regulate Callus Formation in Rice.Rice (New York, N.Y.) · 2025Article
- Genetic diversity of grain yield traits and identification of a grain weight gene SiTGW6 in foxtail millet.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Effect of ABA Pre-Treatment on Rice Plant Transcriptome Response to Multiple Abiotic Stress.Biomolecules · 2023Article
- Wheat ABA Receptor TaPYL5 Constitutes a Signaling Module with Its Downstream Partners TaPP2C53/TaSnRK2.1/TaABI1 to Modulate Plant Drought Response.International journal of molecular sciences · 2023Article
- The rice SnRK family: biological roles and cell signaling modules.Frontiers in plant science · 2023Review
- Candidate Genes and Pathways in Rice Co-Responding to Drought and Salt Identified by gcHap Network.International journal of molecular sciences · 2022Review
- Reconstitution of the Core ABA Signaling in Protoplasts: Transcriptional Activators.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genome-wide association study revealed genetic variations of ABA sensitivity controlled by multiple stress-related genes in rice.Stress biology · 2021Article
- OsMADS23 phosphorylated by SAPK9 confers drought and salt tolerance by regulating ABA biosynthesis in rice.PLoS genetics · 2021Article
- Siberian Wildrye (Plants (Basel, Switzerland) · 2021Article
- TheInternational journal of molecular sciences · 2021Article
- Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize.BMC plant biology · 2021Article
- Reconstitution of Cytokinin Signaling in Rice Protoplasts.International journal of molecular sciences · 2021Article
- OsPP2C09 Is a Bifunctional Regulator in Both ABA-Dependent and Independent Abiotic Stress Signaling Pathways.International journal of molecular sciences · 2021Article
- Genome-wide identification and characterization of ABA receptor PYL gene family in rice.BMC genomics · 2020Article
- Chemical Manipulation of Abscisic Acid Signaling: A New Approach to Abiotic and Biotic Stress Management in Agriculture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice.International journal of molecular sciences · 2020Article
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Authors and funding
10 authors.
Funding
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Abstract
The core components of ABA-dependent gene expression signaling have been identified in Arabidopsis and rice. This signaling pathway consists of four major components; group A OsbZIPs, SAPKs, subclass A OsPP2Cs and OsPYL/RCARs in rice. These might be able to make thousands of combinations through interaction networks resulting in diverse signaling responses. We tried to characterize those gene functions using transient gene expression for rice protoplasts (TGERP) because it is instantaneous and convenient system. Firstly, in order to monitor the ABA signaling output, we developed reporter system named pRab16A-fLUC which consists of Rab16A promoter of rice and luciferase gene. It responses more rapidly and sensitively to ABA than pABRC3-fLUC that consists of ABRC3 of HVA1 promoter in TGERP. We screened the reporter responses for over-expression of each signaling components from group A OsbZIPs to OsPYL/RCARs with or without ABA in TGERP. OsbZIP46 induced reporter most strongly among OsbZIPs tested in the presence of ABA. SAPKs could activate the OsbZIP46 even in the ABA independence. Subclass A OsPP2C6 and -8 almost completely inhibited the OsbZIP46 activity in the different degree through the SAPK9. Lastly, OsPYL/RCAR2 and -5 rescued the OsbZIP46 activity in the presence of SAPK9 and OsPP2C6 dependent on ABA concentration and expression level. By using TGERP, we could characterize successfully the effects of ABA dependent gene expression signaling components in rice. In conclusion, TGERP represents very useful technology to study systemic functional genomics in rice or other monocots.
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