ArticleFrontiers in microbiology2015
The two-component system CpxR/A represses the expression of Salmonella virulence genes by affecting the stability of the transcriptional regulator HilD.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 48 citations in OpenAlex.
- Indispensable role of AcrEF in modulating Salmonella virulence and disrupting host tight junctions to facilitate paracellular entry and invasion.Biology direct · 2025Article
- Salmonella stress response systems as targets for anti-virulence strategies.BMC microbiology · 2025Article
- The two-component system CpxAR controls biofilm formation by directly regulating the T3SS needle tip protein EseB inApplied and environmental microbiology · 2025Article
- Regulatory role of the Cpx ESR in bacterial behaviours.Virulence · 2024Review
- Detection ofAntibiotics (Basel, Switzerland) · 2024Article
- Roles of DJ41_1407 and DJ41_1408 inInternational journal of molecular sciences · 2024Article
- Antimicrobial Resistance: Two-Component Regulatory Systems and Multidrug Efflux Pumps.Antibiotics (Basel, Switzerland) · 2023Review
- Molecular insights into Escherichia coli Cpx envelope stress response activation by the sensor lipoprotein NlpE.Molecular microbiology · 2023Article
- Bacterial virulence regulation through soluble peptidoglycan fragments sensing and response: knowledge gaps and therapeutic potential.FEMS microbiology reviews · 2023Article
- Structure, Substrate Specificity and Role of Lon Protease in Bacterial Pathogenesis and Survival.International journal of molecular sciences · 2023Review
- The Two-Component System CpxRA Represses Salmonella Pathogenicity Island 2 by Directly Acting on theMicrobiology spectrum · 2022Article
- Characterization of Two-Component System CitB Family inInternational journal of molecular sciences · 2022Article
- Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence forInternational journal of molecular sciences · 2021Article
- An incoherent feedforward loop formed by SirA/BarA, HilE and HilD is involved in controlling the growth cost of virulence factor expression by Salmonella Typhimurium.PLoS pathogens · 2021Article
- Article
- Role ofFrontiers in microbiology · 2021Article
- New Roles for Two-Component System Response Regulators ofMicroorganisms · 2020Article
- The Salmonella Typhimurium InvF-SicA complex is necessary for the transcription of sopB in the absence of the repressor H-NS.PloS one · 2020Article
- The CpxR regulates type VI secretion system 2 expression and facilitates the interbacterial competition activity and virulence of avian pathogenic Escherichia coli.Veterinary research · 2019Article
- Transcriptional Sequencing Uncovers Survival Mechanisms of Salmonella enterica Serovar Enteritidis in Antibacterial Egg White.mSphere · 2019Article
Corrections and comments
- Retraction · 2025-02-02Concerns/Issues about Data · Concerns/Issues about Image · Duplication of Data · Investigation by Journal/Publisher · Investigation by Third Party · Manipulation of Images · Unreliable Data · Unreliable Results and/or Conclusions · · See also: https://pubpeer.com/publications/F8153DA54B38437EA92ED30CE7C91C
- Retracted
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica can cause intestinal or systemic infections in humans and animals mainly by the presence of pathogenicity islands SPI-1 and SPI-2, containing 39 and 44 genes, respectively. The AraC-like regulator HilD positively controls the expression of the SPI-1 genes, as well as many other Salmonella virulence genes including those located in SPI-2. A previous report indicates that the two-component system CpxR/A regulates the SPI-1 genes: the absence of the sensor kinase CpxA, but not the absence of its cognate response regulator CpxR, reduces their expression. The presence and absence of cell envelope stress activates kinase and phosphatase activities of CpxA, respectively, which in turn controls the level of phosphorylated CpxR (CpxR-P). In this work, we further define the mechanism for the CpxR/A-mediated regulation of SPI-1 genes. The negative effect exerted by the absence of CpxA on the expression of SPI-1 genes was counteracted by the absence of CpxR or by the absence of the two enzymes, AckA and Pta, which render acetyl-phosphate that phosphorylates CpxR. Furthermore, overexpression of the lipoprotein NlpE, which activates CpxA kinase activity on CpxR, or overexpression of CpxR, repressed the expression of SPI-1 genes. Thus, our results provide several lines of evidence strongly supporting that the absence of CpxA leads to the phosphorylation of CpxR via the AckA/Pta enzymes, which represses both the SPI-1 and SPI-2 genes. Additionally, we show that in the absence of the Lon protease, which degrades HilD, the CpxR-P-mediated repression of the SPI-1 genes is mostly lost; moreover, we demonstrate that CpxR-P negatively affects the stability of HilD and thus decreases the expression of HilD-target genes, such as hilD itself and hilA, located in SPI-1. Our data further expand the insight on the different regulatory pathways for gene expression involving CpxR/A and on the complex regulatory network governing virulence in Salmonella.
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