ArticleJournal of neuroscience methods2015
Combining micro-computed tomography with histology to analyze biomedical implants for peripheral nerve repair.
Article in Journal of neuroscience methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- An X-ray micro-CT protocol enables non-destructive 3D visualization of glandular architecture in archived paraffin-embedded prostate cancer tissue.The journal of pathology. Clinical research · 2026Article
- From pulp to cementum: 3D visualization of soft and hard dental tissues using different ex vivo nano-CT contrast-enhancement techniques.International endodontic journal · 2025Article
- Micro-computed Tomography in the Evaluation of Eosin-stained Axillary Lymph Node Biopsies of Females Diagnosed with Breast Cancer.Scientific reports · 2024Article
- Article
- Nerve Regeneration with a Scaffold Incorporating an Absorbable Zinc-2% Iron Alloy Filament to Improve Axonal Guidance.Pharmaceutics · 2023Article
- Effects of Altering Magnesium Metal Surfaces on Degradation In Vitro and In Vivo during Peripheral Nerve Regeneration.Materials (Basel, Switzerland) · 2023Article
- A pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens.Scientific reports · 2022Article
- Comparative hard x-ray tomography for virtual histology of zebrafish larva, human tooth cementum, and porcine nerve.Journal of medical imaging (Bellingham, Wash.) · 2022Article
- Multiscale imaging of the rat brain using an integrated diceCT and histology workflow.Brain structure & function · 2021Article
- Enhancing CT imaging: A safe protocol to stain and de-stain rare fetal museum specimens using diffusible iodine-based staining (diceCT).Journal of anatomy · 2021Article
- Human fetal whole-body postmortem microfocus computed tomographic imaging.Nature protocols · 2021Article
- Lugol's solution but not formaldehyde affects bone microstructure and bone mineral density parameters at the insertion site of the rotator cuff in rats.Journal of orthopaedic surgery and research · 2021Article
- A New Pipeline to Automatically Segment and Semi-Automatically Measure Bone Length on 3D Models Obtained by Computed Tomography.Frontiers in cell and developmental biology · 2021Article
- ViceCT and whiceCT for simultaneous high-resolution visualization of craniofacial, brain and ventricular anatomy from micro-computed tomography.Scientific reports · 2020Article
- Article
- Three-dimensional architecture of human diabetic peripheral nerves revealed by X-ray phase contrast holographic nanotomography.Scientific reports · 2020Article
- Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects:Neural regeneration research · 2019Article
- Imaging in the repair of peripheral nerve injury.Nanomedicine (London, England) · 2019Article
- Imaging of Mouse Brain Fixated in Ethanol in Micro-CT.BioMed research international · 2019Article
- Investigating the microstructure of plant leaves in 3D with lab-based X-ray computed tomography.Plant methods · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundBiomedical implants used in tissue engineering repairs, such as scaffolds to repair peripheral nerves, can be too large to examine completely with histological analyses. Micro-computed tomography (micro-CT) with contrast agents allows ex vivo visualization of entire biomaterial implants and their interactions with tissues, but contrast agents can interfere with histological analyses of the tissues or cause shrinkage or loss of antigenicity. NEW
methodSoft tissue, ex vivo micro-CT imaging using Lugol's iodine was compatible with histology after using a rapid (48 h) method of removing iodine.
resultsAdult normal and repaired rat sciatic nerves were infiltrated ex vivo with iodine, imaged with micro-CT and then the iodine was removed by incubating tissues in sodium thiosulfate. Subsequent paraffin sections of normal nerve tissues showed no differences in staining with hematoxylin and eosin or immunostaining with multiple antibodies. Iodine treatment and removal did not alter axonal diameter, nuclear size or relative area covered by immunostained axons (p>0.05). Combining imaging modalities allowed comparisons of macroscopic and microscopic features of nerve tissues regenerating through simple nerve conduits or nerve conduits containing a titanium wire for guidance. COMPARISON WITH EXISTING
methodsQuantification showed that treatment with iodine and sodium thiosulfate did not result in tissue shrinkage or loss of antigenicity.
conclusionsBecause this combination of treatments is rapid and does not alter tissue morphology, this expands the ex vivo methods available to examine the success of biomaterial implants used for tissue engineering repairs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.