ArticleJournal of industrial microbiology & biotechnology2015
Expression of plectasin in Bacillus subtilis using SUMO technology by a maltose-inducible vector.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Recombinant Expression and Antimicrobial Mechanism of Cysteine-Rich Antimicrobial Peptides fromMarine drugs · 2026Article
- Heterologous Expression and Antimicrobial Targets of a Novel Glycine-Rich Antimicrobial Peptide fromMarine drugs · 2025Article
- Heterologous Expression and Antimicrobial Mechanism of a Cysteine-Rich Peptide from BarnacleMicroorganisms · 2025Article
- Screening and heterologous expression of an antimicrobial peptide SCAK33 with broad-spectrum antimicrobial activity resourced from sea cucumber proteome.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Antimicrobial Peptides: Mechanism, Expressions, and Optimization Strategies.Probiotics and antimicrobial proteins · 2025Review
- High-level biosynthesis and purification of the antimicrobial peptide Kiadin based on non-chromatographic purification and acid cleavage methods.Biotechnology for biofuels and bioproducts · 2025Article
- Unlocking the power of antimicrobial peptides: advances in production, optimization, and therapeutics.Frontiers in cellular and infection microbiology · 2025Review
- The future of recombinant host defense peptides.Microbial cell factories · 2022Review
- Boosting expression level of plectasin in recombinant Pichia pastoris via 2A self-processing peptide assembly.Applied microbiology and biotechnology · 2022Article
- A eukaryotic expression strategy for producing the novel antimicrobial peptide PRW4.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Nicotinamide phosphoribosyltransferase purification using SUMO expression system.Analytical biochemistry · 2020Article
- Antifungal Peptides as Therapeutic Agents.Frontiers in cellular and infection microbiology · 2020Review
- The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis.Microbial cell factories · 2019Article
- Expression of soluble native protein inBiotechnology reports (Amsterdam, Netherlands) · 2018Article
- Suppression of the toxicity of Bac7 (1-35), a bovine peptide antibiotic, and its production in E. coli.AMB Express · 2016Article
- Rational Design of a Carrier Protein for the Production of Recombinant Toxic Peptides in Escherichia coli.PloS one · 2016Article
- A novel expression vector for the secretion of abaecin in Bacillus subtilis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plectasin, the first fungus defensin, is especially efficient against Gram-positive bacteria. To explore an effective approach for expressing plectasin in Bacillus subtilis, the sequence encoding plectasin fused with the small ubiquitin-like modifier (SUMO) gene, the 6 × His gene and the signal peptide of SacB were cloned into an E. coli-B. subtilis shuttle vector pGJ148 in which the maltose utilization operon promoter Pglv directed the expression. The fusion protein successfully secreted in culture and approximately, 41 mg of the recombinant fusion protein SUMO-plectasin was purified per liter of culture supernatant. After purification by Ni-NTA resin column and digestion by SUMO protease, 5.5 mg of plectasin with a purity of 94 % was obtained from 1 L fermentation culture. Recombinant plectasin was found inhibition activity against S. pneumoniae, S. aureus and S. epidermidis. These results indicate that the maltose-induced expression system may be a safe and efficient way for the large-scale production of soluble peptides in B. subtilis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.