ArticleJournal of the American Chemical Society2015
Structural Adaptability Facilitates Histidine Heme Ligation in a Cytochrome P450.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Enzymatic Metal-Hydrogen Atom Transfer with a Cobalt Protoporphyrin Cofactor.Journal of the American Chemical Society · 2026Article
- Large-scale energy decomposition for the analysis of protein stability.Cell stress & chaperones · 2025Article
- Electron Transfer Mechanism at the Interface of Multi-Heme Cytochromes and Metal Oxide.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Tailorable Tetrahelical Bundles as a Toolkit for Redox Studies.The journal of physical chemistry. B · 2022Article
- A novel catalytic heme cofactor in SfmD with a single thioether bond and aChemical science · 2021Article
- Antibacterial Activity of Synthetic Cationic Iron Porphyrins.Antioxidants (Basel, Switzerland) · 2020Article
- Enhanced Activity and Substrate Specificity by Site-Directed Mutagenesis for the P450 119 Peroxygenase Catalyzed Sulfoxidation of Thioanisole.ChemistryOpen · 2019Article
- Selective CH bond functionalization with engineered heme proteins: new tools to generate complexity.Current opinion in chemical biology · 2019Review
- Exploiting and engineering hemoproteins for abiological carbene and nitrene transfer reactions.Current opinion in biotechnology · 2017Review
- Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.Natural product reports · 2017Review
- An artificial metalloenzyme with the kinetics of native enzymes.Science (New York, N.Y.) · 2016Article
- Identification of Mechanism-Based Inactivation in P450-Catalyzed Cyclopropanation Facilitates Engineering of Improved Enzymes.Journal of the American Chemical Society · 2016Article
- Mutating a Highly Conserved Residue in Diverse Cytochrome P450s Facilitates Diastereoselective Olefin Cyclopropanation.Chembiochem : a European journal of chemical biology · 2016Article
- Preface.Methods in enzymology · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Almost all known members of the cytochrome P450 (CYP) superfamily conserve a key cysteine residue that coordinates the heme iron. Although mutation of this residue abolishes monooxygenase activity, recent work has shown that mutation to either serine or histidine unlocks non-natural carbene- and nitrene-transfer activities. Here we present the first crystal structure of a histidine-ligated P450. The T213A/C317H variant of the thermostable CYP119 from Sulfolobus acidocaldarius maintains heme iron coordination through the introduced ligand, an interaction that is accompanied by large changes in the overall protein structure. We also find that the axial cysteine C317 may be substituted with any other amino acid without abrogating folding and heme cofactor incorporation. Several of the axial mutants display unusual spectral features, suggesting that they have active sites with unique steric and electronic properties. These novel, highly stable enzyme active sites will be fruitful starting points for investigations of non-natural P450 catalysis and mechanisms.
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