ArticleCryobiology2015
The crucial role of zona pellucida in cryopreservation of oocytes by vitrification.
Article in Cryobiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Research Progress on Mammalian Oocyte Vitrification: From Damage Mechanisms to Optimization Strategies.Animals : an open access journal from MDPI · 2026Review
- Oocyte-inspired universal whole-cell vaccines against tumor heterogeneity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Atomic Force Microscopy Reveals Differences In Mechanical Properties Linked To Cortical Structure In Mouse And Human Oocytes.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Mechanical Characterization of Murine Oocytes by Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Embryological Characteristics and Clinical Outcomes of Oocytes with Heterogeneous Zona Pellucida During Assisted Reproduction Treatment: A Retrospective Study.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes.Microsystems & nanoengineering · 2020Article
- Study of the mechanical properties of fresh and cryopreserved individual human oocytes.European biophysics journal : EBJ · 2019Article
- Dual Suppression Effect of Magnetic Induction Heating and Microencapsulation on Ice Crystallization Enables Low-Cryoprotectant Vitrification of Stem Cell-Alginate Hydrogel Constructs.ACS applied materials & interfaces · 2018Article
- Article
- Bio-inspired solute enables preservation of human oocytes using minimum volume vitrification.Journal of tissue engineering and regenerative medicine · 2018Article
- Hydrogel Encapsulation Facilitates Rapid-Cooling Cryopreservation of Stem Cell-Laden Core-Shell Microcapsules as Cell-Biomaterial Constructs.Advanced healthcare materials · 2017Article
- Scalable Production and Cryostorage of Organoids Using Core-Shell Decoupled Hydrogel Capsules.Advanced biosystems · 2017Article
- Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development.Clinical and experimental reproductive medicine · 2017Article
- Microscale Biomaterials with Bioinspired Complexity of Early Embryo Development and in the Ovary for Tissue Engineering and Regenerative Medicine.ACS biomaterials science & engineering · 2017Article
- Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification.Acta biomaterialia · 2016Article
- Alginate Hydrogel Microencapsulation Inhibits Devitrification and Enables Large-Volume Low-CPA Cell Vitrification.Advanced functional materials · 2015Article
- Microfluidics for cryopreservation.Biotechnology advancesReview
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Mammalian oocytes have a proteinaceous hydrogel-like outer shell known as the zona pellucida (ZP) that semi-encloses their plasma membrane and cytoplasm. In this study, we cryopreserved mouse oocytes either with or without ZP by vitrification. Our results show that the presence of an intact ZP could significantly improve the post-vitrification survival of oocytes to 92.1% from 13.3% for oocytes without ZP. Moreover, there was no significant difference in embryonic development between fresh and cryopreserved oocytes with ZP after in vitro fertilization (IVF). Further atomic force microscopy (AFM) analysis showed that the intact oocytes with ZP have an elastic modulus that is more than 85 times higher than that of oocytes without ZP. This may partially explain the important role of ZP in protecting the oocytes by resisting the mechanical stress due to possible ice formation during cryopreservation by vitrification. Collectively, this study reveals a new biophysical role of ZP during vitrification of oocytes and suggests microencapsulation of the many mammalian cells without a ZP in ZP-like hydrogel is an effective strategy to improve their survival post cryopreservation by vitrification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.