ArticleJournal of the American Society for Mass Spectrometry2015
Homogeneous matrix deposition on dried agar for MALDI imaging mass spectrometry of microbial cultures.
Article in Journal of the American Society for Mass Spectrometry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.Nature reviews. Methods primers · 2026Article
- Use of analytical strategies to understand spatial chemical variation in bacterial surface communities.Journal of bacteriology · 2025Review
- Rapid and Robust Workflows Using Different Ionization, Computation, and Visualization Approaches for Spatial Metabolome Profiling of Microbial Natural Products inACS measurement science au · 2024Article
- Bacteria from the Amycolatopsis genus associated with a toxic bird secrete protective secondary metabolites.Nature communications · 2024Article
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- Streptomyces polyketides mediate bacteria-fungi interactions across soil environments.Nature microbiology · 2023Article
- Review
- The Exploration of Microbial Natural Products and Metabolic Interaction Guided by Mass Spectrometry Imaging.Bioengineering (Basel, Switzerland) · 2022Review
- Spray-Based Application of Matrix to Agar-Based Microbial Samples for Reproducible Sample Adherence in MALDI MSI.Journal of the American Society for Mass Spectrometry · 2022Article
- Utilizing imaging mass spectrometry to analyze microbial biofilm chemical responses to exogenous compounds.Methods in enzymology · 2022Article
- Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging.International journal of molecular sciences · 2021Review
- Expanding Molecular Coverage in Mass Spectrometry Imaging of Microbial Systems Using Metal-Assisted Laser Desorption/Ionization.Microbiology spectrum · 2021Article
- Imaging mass spectrometry for natural products discovery: a review of ionization methods.Natural product reports · 2020Article
- Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry.Journal of visualized experiments : JoVE · 2019Article
- In plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids.Nature microbiology · 2019Article
- High-resolution atmospheric-pressure MALDI mass spectrometry imaging workflow for lipidomic analysis of late fetal mouse lungs.Scientific reports · 2019Article
- Imaging Mass Spectrometry Reveals Crosstalk between the Fallopian Tube and the Ovary that Drives Primary Metastasis of Ovarian Cancer.ACS central science · 2018Article
- Quantitative SIMS Imaging of Agar-Based Microbial Communities.Analytical chemistry · 2018Article
- Mass Spectrometry Imaging: A Review of Emerging Advancements and Future Insights.Analytical chemistry · 2018Review
- Antagonistic bacteria disrupt calcium homeostasis and immobilize algal cells.Nature communications · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix deposition on agar-based microbial colonies for MALDI imaging mass spectrometry is often complicated by the complex media on which microbes are grown. This Application Note demonstrates how consecutive short spray pulses of a matrix solution can form an evenly closed matrix layer on dried agar. Compared with sieving dry matrix onto wet agar, this method supports analyte cocrystallization, which results in significantly more signals, higher signal-to-noise ratios, and improved ionization efficiency. The even matrix layer improves spot-to-spot precision of measured m/z values when using TOF mass spectrometers. With this technique, we established reproducible imaging mass spectrometry of myxobacterial cultures on nutrient-rich cultivation media, which was not possible with the sieving technique. Graphical Abstract ᅟ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.