Evidence map›Paper›PMID 26297175›Full record

ArticleCurrent microbiology2015

Determinants of Proteolysis and Cell-Binding for the Shigella flexneri Cytotoxin, SigA.

Eng Guan Chua, Keith Al-Hasani, Martin Scanlon, Ben Adler, Harry Sakellaris

Abstract read
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In one paragraph

Article in Current microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. SepA EnhancesmBio · 2021
    Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Eng Guan ChuaSchool of Pathology and Laboratory Medicine, University of Western Australia, Nedlands, WA, 6009, Australia.
Keith Al-HasaniDepartment of Microbiology, Monash University, Clayton, VIC, 3800, Australia.
Martin ScanlonMedicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, VIC, 3052, Australia.
Ben AdlerDepartment of Microbiology, Monash University, Clayton, VIC, 3800, Australia.
Harry SakellarisSchool of Pathology and Laboratory Medicine, University of Western Australia, Nedlands, WA, 6009, Australia. h.sakellaris@griffith.edu.au.
Monash University · AUGriffith University · AUUniversity of Western Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Shigella flexneri secretes an enterotoxic, SPATE family autotransporter (AT), SigA, which has cytopathic activity towards cultured epithelial cells. Its cytopathic activity is due to its ability to degrade the cytoskeletal protein, α-fodrin. The mechanisms by which AT toxins target cells and tissues differ and the details of how SigA acts are not known. In the current study, the determinants of proteolysis and cell-targeting for SigA were determined. We demonstrate that the SigA passenger or α-domain consists of two functionally distinct domains, designated α1 and α2, which are sufficient to specify proteolytic and cell-binding activities, respectively.

Indexed as

Bacterial ToxinsCell LineCytotoxinsEscherichia coliGene ExpressionProtein Interaction Domains and MotifsProteolysisRecombinant Fusion ProteinsSequence DeletionShigella flexneriBacterial ToxinsCytotoxinsRecombinant Fusion Proteins

Identifiers

PMID26297175
OpenAlexW1793691475

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.