ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2015
Mature Surfactant Protein-B Expression by Immunohistochemistry as a Marker for Surfactant System Development in the Fetal Sheep Lung.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Maternal high-fat high-energy diet impairs surfactant maturation in the non-human primate fetal lung.International journal of obesity (2005) · 2026Article
- Plasma-Derived Extracellular Vesicle-Enriched Fractions as a Potential Source of Biomarkers for Systemic Sclerosis-Associated Interstitial Lung Disease (SSc-ILD): A Shotgun Proteomic Exploration Analysis.Diagnostics (Basel, Switzerland) · 2026Article
- Moderate maternal nutrient restriction alters type II alveolar epithelial cell density in the non-human primate fetal lung.Experimental physiology · 2026Article
- Antenatal betamethasone impairs markers of cardiac development and function in near-term lambs.Experimental physiology · 2026Article
- Elevated cortisol concentration in preterm sheep fetuses impacts heart development.Experimental physiology · 2025Article
- Sex-specific effects of betamethasone on glucocorticoid and apoptotic signalling pathways in the sheep placenta.The Journal of physiology · 2025Article
- Antenatal Vitamin C differentially affects lung development in normally grown and growth restricted sheep.Pediatric research · 2025Article
- Betamethasone improved near-term neonatal lamb lung maturation in experimental maternal asthma.Experimental physiology · 2024Article
- Chronic fetal hypoxia and antenatal Vitamin C exposure differentially regulate molecular signalling in the lung of female lambs in early adulthood.Frontiers in physiology · 2024Article
- Molecular regulation of lung maturation in near-term fetal sheep by maternal daily vitamin C treatment in late gestation.Pediatric research · 2022Article
- The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.PloS one · 2021Article
- Differential gene responses 3 days following infarction in the fetal and adolescent sheep heart.Physiological genomics · 2020Article
- Identification of Novel miRNAs Involved in Cardiac Repair Following Infarction in Fetal and Adolescent Sheep Hearts.Frontiers in physiology · 2020Article
- Differential Response to Injury in Fetal and Adolescent Sheep Hearts in the Immediate Post-myocardial Infarction Period.Frontiers in physiology · 2019Article
- Differential effects of late gestation maternal overnutrition on the regulation of surfactant maturation in fetal and postnatal life.The Journal of physiology · 2017Article
- Maternal chronic hypoxia increases expression of genes regulating lung liquid movement and surfactant maturation in male fetuses in late gestation.The Journal of physiology · 2017Article
- Structural and molecular regulation of lung maturation by intratracheal vascular endothelial growth factor administration in the normally grown and placentally restricted fetus.The Journal of physiology · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Evaluation of the number of type II alveolar epithelial cells (AECs) is an important measure of the lung's ability to produce surfactant. Immunohistochemical staining of these cells in lung tissue commonly uses antibodies directed against mature surfactant protein (SP)-C, which is regarded as a reliable SP marker of type II AECs in rodents. There has been no study demonstrating reliable markers for surfactant system maturation by immunohistochemistry in the fetal sheep lung despite being widely used as a model to study lung development. Here we examine staining of a panel of surfactant pro-proteins (pro-SP-B and pro-SP-C) and mature proteins (SP-B and SP-C) in the fetal sheep lung during late gestation in the saccular/alveolar phase of development (120, 130, and 140 days), with term being 150 ± 3 days, to identify the most reliable marker of surfactant producing cells in this species. Results from this study indicate that during late gestation, use of anti-SP-B antibodies in the sheep lung yields significantly higher cell counts in the alveolar epithelium than SP-C antibodies. Furthermore, this study highlights that mature SP-B antibodies are more reliable markers than SP-C antibodies to evaluate surfactant maturation in the fetal sheep lung by immunohistochemistry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.