Evidence map›Paper›PMID 26296527›Full record

ArticleProtein & cell2015

Dermal fibroblast expression of stromal cell-derived factor-1 (SDF-1) promotes epidermal keratinocyte proliferation in normal and diseased skin.

Chunji Quan, Moon Kyun Cho, Yuan Shao, Laurel E Mianecki, Eric Liao, Daniel Perry, Taihao Quan

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein & cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.

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  10. The Central Roles of Keratinocytes in Coordinating Skin Immunity.The Journal of investigative dermatology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

Chunji QuanDepartment of Pathology, Affiliated Hospital of Yanbian University, Yanji, 133000, China.
Moon Kyun ChoDepartment of Dermatology, Soonchunhyang University College of Medicine, Seoul, Republic of Korea.
Yuan ShaoHollings Cancer Center Biorepository and Tissue Analysis Resource, Medical University of South Carolina, Charleston, SC, 29425, USA.
Laurel E MianeckiDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA.
Eric LiaoDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA.
Daniel PerryDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA.
Taihao QuanDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA. thquan@umich.edu.
University of Michigan–Ann Arbor · USMUSC Hollings Cancer Center · USSoonchunhyang University · KRYanbian University · CN

Funding

CYR61-a novel potential mediator of photoaged human skinR01ES014697 · NIEHS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI QUAN, TAIHAO · 2007 to 2010
$1.7M
NIEHS NIH HHS ES014697NIEHS NIH HHS ES014697 30S1
6 · The paper itself

Abstract

Stromal cells provide a crucial microenvironment for overlying epithelium. Here we investigated the expression and function of a stromal cell-specific protein, stromal cell-derived factor-1 (SDF-1), in normal human skin and in the tissues of diseased skin. Immunohistology and laser capture microdissection (LCM)-coupled quantitative real-time RT-PCR revealed that SDF-1 is constitutively and predominantly expressed in dermal stromal cells in normal human skin in vivo. To our surprise, an extremely high level of SDF-1 transcription was observed in the dermis of normal human skin in vivo, evidenced by much higher mRNA expression level than type I collagen, the most abundant and highly expressed protein in human skin. SDF-1 was also upregulated in the tissues of many human skin disorders including psoriasis, basal cell carcinoma (BCC), and squamous cell carcinoma (SCC). Double immunostaining for SDF-1 and HSP47 (heat shock protein 47), a marker of fibroblasts, revealed that fibroblasts were the major source of stroma-cell-derived SDF-1 in both normal and diseased skin. Functionally, SDF-1 activates the ERK (extracellular-signal-regulated kinases) pathway and functions as a mitogen to stimulate epidermal keratinocyte proliferation. Both overexpression of SDF-1 in dermal fibroblasts and treatment with rhSDF-1 to the skin equivalent cultures significantly increased the number of keratinocyte layers and epidermal thickness. Conversely, the stimulative function of SDF-1 on keratinocyte proliferation was nearly completely eliminated by interfering with CXCR4, a specific receptor of SDF-1, or by knock-down of SDF-1 in fibroblasts. Our data reveal that extremely high levels of SDF-1 provide a crucial microenvironment for epidermal keratinocyte proliferation in both physiologic and pathologic skin conditions.

Indexed as

Epidermal CellsGene Expression RegulationAdultCell ProliferationChemokine CXCL12EpidermisExtracellular Signal-Regulated MAP KinasesFibroblastsHumansKeratinocytesSignal TransductionSkin DiseasesChemokine CXCL12Extracellular Signal-Regulated MAP Kinasesdermal fibroblastkeratinocyteproliferationpsoriasisSDF-1skin cancer

Identifiers

PMID26296527
PMCPMC4656211
OpenAlexW1663577648

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.