ArticleMicrobial cell factories2015
Developing a high-throughput screening method for threonine overproduction based on an artificial promoter.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 72 citations in OpenAlex.
- Combining biosensor and metabolic network optimization strategies for enhanced L-threonine production in Escherichia coli.Biotechnology for biofuels and bioproducts · 2025Article
- Engineering of L-threonine and L-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening.Bioresources and bioprocessing · 2025Article
- High Throughput Screening of Transcription Factor LysG for Constructing a Better Lysine Biosensor.Biosensors · 2024Article
- Have you tried turning it off and on again? Oscillating selection to enhance fitness-landscape traversal in adaptive laboratory evolution experiments.Metabolic engineering communications · 2023Review
- Transcription factor-based biosensors for screening and dynamic regulation.Frontiers in bioengineering and biotechnology · 2023Review
- Recent trends in biocatalysis.Chemical Society reviews · 2021Review
- Development of a Hyperosmotic Stress Inducible Gene Expression System by Engineering the MtrA/MtrB-DependentFrontiers in microbiology · 2021Article
- Inference of Regulatory System for TAG Biosynthesis inBioengineering (Basel, Switzerland) · 2020Article
- Discovery and identification of medium-chain fatty acid responsive promoters inEngineering in life sciences · 2020Article
- Improvement of l-Valine Production by Atmospheric and Room Temperature Plasma Mutagenesis and High-Throughput Screening inACS omega · 2020Article
- Expression regulation of multiple key genes to improve L-threonine in Escherichia coli.Microbial cell factories · 2020Article
- Increasing L-threonine production in Escherichia coli by overexpressing the gene cluster phaCAB.Journal of industrial microbiology & biotechnology · 2019Article
- Tools and systems for evolutionary engineering of biomolecules and microorganisms.Journal of industrial microbiology & biotechnology · 2019Review
- GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli.Microbial cell factories · 2019Article
- Heavy-ion mutagenesis significantly enhances enduracidin production byEngineering in life sciences · 2019Article
- Enhanced production of l-lactic acid byEngineering in life sciences · 2018Article
- The Applications of Promoter-gene-Engineered Biosensors.Sensors (Basel, Switzerland) · 2018Review
- In vivo biosensors: mechanisms, development, and applications.Journal of industrial microbiology & biotechnology · 2018Article
- A Novel Corynebacterium glutamicum l-Glutamate Exporter.Applied and environmental microbiology · 2018Article
- Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundL-Threonine is an important amino acid for animal feed. Though the industrial fermentation technology of threonine achieved a very high level, there is still significant room to further improve the industrial strains. The biosensor-based high-throughput screening (HTS) technology has demonstrated its powerful applications. Unfortunately, for most of valuable fine chemicals such as threonine, a HTS system has not been established mainly due to the absence of a suitable biosensor. In this study, we developed a HTS method to gain high-yielding threonine-producing strains.
resultsNovel threonine sensing promoters including cysJp and cysHp were discovered by proteomic analyses of Escherichia coli in response to extracellular threonine challenges. The HTS method was constructed using a device composed of the fused cysJp and cysHp as a promoter and a linked enhanced green fluorescent protein gene as a reporter. More than 400 strains were selected with fluorescence activated cell sorting technology from a library of 20 million mutants and tested within 1 week. Thirty-four mutants have higher productivities than the starting industrial producer. One mutant produced 17.95 % more threonine in a 5-L jar fermenter.
conclusionsThis method should play a functional role for continuous improvement of threonine industry. Additionally, the threonine sensor construction using promoters obtained by proteomics analyses is so convenient that it would be easily extended to develop HTS models for other biochemicals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.