ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2016
Effect of Laminin-A4 inhibition on cluster formation of human osteoarthritic chondrocytes.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Disease-Associated Signatures Persist in Extracellular Vesicles from Reprogrammed Cells of Osteoarthritis Patients.International journal of molecular sciences · 2025Article
- Single-cell transcriptome and crosstalk analysis reveals immune alterations and key pathways in the bone marrow of knee OA patients.iScience · 2024Article
- Chemically programmed metabolism drives a superior cell fitness for cartilage regeneration.Science advances · 2024Article
- Transcriptomic changes in porcine articular cartilage one year following disruption of the anterior cruciate ligament.PloS one · 2023Article
- The tendon interfascicular basement membrane provides a vascular niche for CD146+ cell subpopulations.Frontiers in cell and developmental biology · 2022Article
- The Role of Extracellular Matrix Expression, ERK1/2 Signaling and Cell Cohesiveness for Cartilage Yield from iPSCs.International journal of molecular sciences · 2019Article
- Down-regulated LAMA4 inhibits oxidative stress-induced apoptosis of retinal ganglion cells through the MAPK signaling pathway in rats with glaucoma.Cell cycle (Georgetown, Tex.) · 2019Article
- Genetic variation of aggrecanase-2 (ADAMTS5) in susceptibility to osteoarthritis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2019Article
- A Meta-Analysis of Non-Osteoarthritis and Osteoarthritis Chondrocyte Gene Expression to Determine the Efficacy of Autologous Chondrocyte Transplantation as a Viable Treatment Option.Medical case reports and short reviews · 2019Article
- Article
- The role of laminins in cartilaginous tissues: from development to regeneration.European cells & materials · 2017Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 3 countries.
Funding
Abstract
Formation of chondrocyte clusters is not only a morphological sign of osteoarthritis but it is also observed in cell culture. Active locomotion of chondrocytes is controlled by integrins in vitro. Integrins bind to Laminin-A4 (LAMA4), a protein that is highly expressed in vivo in clusters of hypertrophic chondrocytes. We tested if LAMA4 is relevant for cluster formation. Human chondrocytes were cultured in a 2D matrigel model and treated with different concentrations of a monoclonal inhibitory anti-LAMA4-antibody. Migration and cluster formation was analysed using live cell imaging technique. Full genome gene expression analysis was performed to assess the effect of LAMA4 inhibition. The data set were screened for genes relevant to cell motility. F-actin staining was performed to document cytoskeletal changes. Anti-LAMA4 treatment significantly reduced the rate of cluster formation in human chondrocytes. Cells changed their surface morphology and exhibited fewer protrusions. Expression of genes associated with cellular motility and migration was affected by anti-LAMA4 treatment. LAMA4-integrin signalling affects chondrocyte morphology and gene expression in vitro, thereby contributing to cluster formation in human osteoarthritic chondrocytes.
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