Evidence map›Paper›PMID 26294422›Full record

ArticleCirculation. Heart failure2015

Cardiac Resynchronization Therapy Reduces Subcellular Heterogeneity of Ryanodine Receptors, T-Tubules, and Ca2+ Sparks Produced by Dyssynchronous Heart Failure.

Hui Li, Justin G Lichter, Thomas Seidel, Gordon F Tomaselli, John H B Bridge, Frank B Sachse

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation. Heart failure, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
5.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 46 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Heart failure study of multipoint pacing effects on ventriculoarterial coupling: Rationale and design of the HUMVEE trial.Annals of noninvasive electrocardiology : the official journal of the International Society for Holter and Noninvasive Electrocardiology, Inc · 2018
    Observational
  12. Review
  13. New Insights in Cardiac Calcium Handling and Excitation-Contraction Coupling.Advances in experimental medicine and biology · 2018
    Review
  14. Article
  15. Review
  16. Review
  17. Review
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Hui LiFrom the Nora Eccles Harrison Cardiovascular Research and Training Institute (H.L., J.G.L., T.S., J.H.B., F.B.S.), and Department of Bioengineering (J.G.L., F.B.S.), University of Utah, Salt Lake City; and Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD (G.F.T.).
Justin G LichterFrom the Nora Eccles Harrison Cardiovascular Research and Training Institute (H.L., J.G.L., T.S., J.H.B., F.B.S.), and Department of Bioengineering (J.G.L., F.B.S.), University of Utah, Salt Lake City; and Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD (G.F.T.).
Thomas SeidelFrom the Nora Eccles Harrison Cardiovascular Research and Training Institute (H.L., J.G.L., T.S., J.H.B., F.B.S.), and Department of Bioengineering (J.G.L., F.B.S.), University of Utah, Salt Lake City; and Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD (G.F.T.).
Gordon F TomaselliFrom the Nora Eccles Harrison Cardiovascular Research and Training Institute (H.L., J.G.L., T.S., J.H.B., F.B.S.), and Department of Bioengineering (J.G.L., F.B.S.), University of Utah, Salt Lake City; and Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD (G.F.T.).
John H B BridgeFrom the Nora Eccles Harrison Cardiovascular Research and Training Institute (H.L., J.G.L., T.S., J.H.B., F.B.S.), and Department of Bioengineering (J.G.L., F.B.S.), University of Utah, Salt Lake City; and Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD (G.F.T.).
Frank B SachseFrom the Nora Eccles Harrison Cardiovascular Research and Training Institute (H.L., J.G.L., T.S., J.H.B., F.B.S.), and Department of Bioengineering (J.G.L., F.B.S.), University of Utah, Salt Lake City; and Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD (G.F.T.). frank.sachse@utah.edu.
Johns Hopkins University · US

Funding

Pathobiology of Cardiac Dyssynchrony &ResynchronizationP01HL077180 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI VAN EYK, JENNIFER E · 2004 to 2014
$22.8M
Remodeling of the Transverse Tubular System and Ryanodine Receptor Clusters in DyR01HL094464 · NHLBI · UNIVERSITY OF UTAH · PI SACHSE, FRANK BERND · 2010 to 2014
$1.9M
NHLBI NIH HHS P01 HL077180NHLBI NIH HHS R01 HL094464
6 · The paper itself

Abstract

backgroundCardiac resynchronization therapy (CRT) is a major advance for treatment of patients with dyssynchronous heart failure (DHF). However, our understanding of DHF-associated remodeling of subcellular structure and function and their restoration after CRT remains incomplete. METHODS AND

resultsWe investigated subcellular heterogeneity of remodeling of structures and proteins associated with excitation-contraction coupling in cardiomyocytes in DHF and after CRT. Three-dimensional confocal microscopy revealed subcellular heterogeneity of ryanodine receptor (RyR) density and the transverse tubular system (t-system) in a canine model of DHF. RyR density at the ends of lateral left ventricular cardiomyocytes was higher than that in cell centers, whereas the t-system was depleted at cell ends. In anterior left ventricular cardiomyocytes, however, we found a similar degree of heterogeneous RyR remodeling, despite preserved t-system. Synchronous heart failure was associated with marginal heterogeneity of RyR density. We used rapid scanning confocal microscopy to investigate effects of heterogeneous structural remodeling on calcium signaling. In DHF, diastolic Ca(2+) spark density was smaller at cell ends versus centers. After CRT, subcellular heterogeneity of structures and function was reduced.

conclusionsRyR density exhibits remarkable subcellular heterogeneity in DHF. RyR remodeling occurred in lateral and anterior cardiomyocytes, but remodeling of t-system was confined to lateral myocytes. These findings indicate that different mechanisms underlie remodeling of RyRs and t-system. Furthermore, we suggest that ventricular dyssynchrony exacerbates subcellular remodeling in heart failure. CRT efficiently reduced subcellular heterogeneity. These results will help to explain remodeling of excitation-contraction coupling in disease and restoration after CRT.

Indexed as

Cardiac Resynchronization TherapyAnimalsDisease Models, AnimalDogsExcitation Contraction CouplingHeart FailureMicroscopy, ConfocalMyocytes, CardiacRyanodine Receptor Calcium Release ChannelVentricular RemodelingRyanodine Receptor Calcium Release Channelcardiac resynchronization therapyexcitation–contraction couplingheart failuremyocyteremodelingryanodine receptorstransverse tubular system

Identifiers

PMID26294422
PMCPMC4651794
OpenAlexW2122509573

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.