ArticleAnnals of biomedical engineering2016
Cellular and Nuclear Alignment Analysis for Determining Epithelial Cell Chirality.
Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Development of a Stereolithography 3D Printing-Based Micropatterning Method to Study Endothelial-to-Mesenchymal Transition Mechanobiology.Annals of biomedical engineering · 2026Article
- Contractile forces direct the chiral swirling of minimal cell collectives.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells.Advanced healthcare materials · 2024Article
- Asymmetrical positioning of cell organelles reflects the cell chirality of mouse myoblast cells.APL bioengineering · 2024Article
- Helical vasculogenesis driven by cell chirality.Science advances · 2024Article
- Fabrication of Micropatterns of Aligned Collagen Fibrils.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- A toolbox to analyze collective cell migration, proliferation and cellular organization simultaneously.Cell adhesion & migration · 2023Article
- Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width.RSC advances · 2022Article
- Article
- Cell Chirality as a Novel Measure for Cytotoxicity.Advanced biology · 2022Review
- Microtopographical patterns promote different responses in fibroblasts and Schwann cells: A possible feature for neural implants.Journal of biomedical materials research. Part A · 2021Article
- Cell chirality in cardiovascular development and disease.APL bioengineering · 2020Review
- Cell organelle-based analysis of cell chirality.Communicative & integrative biology · 2019Article
- Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Epithelial Cell Chirality Revealed by Three-Dimensional Spontaneous Rotation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cell chirality regulates intercellular junctions and endothelial permeability.Science advances · 2018Article
- Outline-etching image segmentation reveals enhanced cell chirality through intercellular alignment.Biotechnology and bioengineering · 2018Article
- Spontaneous shear flow in confined cellular nematics.Nature physics · 2018Article
- Article
- Cell Chirality Drives Left-Right Asymmetric Morphogenesis.Frontiers in cell and developmental biology · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Left-right (LR) asymmetry is a biologically conserved property in living organisms that can be observed in the asymmetrical arrangement of organs and tissues and in tissue morphogenesis, such as the directional looping of the gastrointestinal tract and heart. The expression of LR asymmetry in embryonic tissues can be appreciated in biased cell alignment. Previously an in vitro chirality assay was reported by patterning multiple cells on microscale defined geometries and quantified the cell phenotype-dependent LR asymmetry, or cell chirality. However, morphology and chirality of individual cells on micropatterned surfaces has not been well characterized. Here, a Python-based algorithm was developed to identify and quantify immunofluorescence stained individual epithelial cells on multicellular patterns. This approach not only produces results similar to the image intensity gradient-based method reported previously, but also can capture properties of single cells such as area and aspect ratio. We also found that cell nuclei exhibited biased alignment. Around 35% cells were misaligned and were typically smaller and less elongated. This new imaging analysis approach is an effective tool for measuring single cell chirality inside multicellular structures and can potentially help unveil biophysical mechanisms underlying cellular chiral bias both in vitro and in vivo.
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Registered trials
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