ArticleScientific reports2015
Imaging Fibrosis and Separating Collagens using Second Harmonic Generation and Phasor Approach to Fluorescence Lifetime Imaging.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 102 citations in OpenAlex.
- Machine Learning-Assisted SHG Morphometry Reveals Distinct Collagen Microarchitectures of Trabecular Bone and Fibrosis in Bone Marrow Biopsies.International journal of molecular sciences · 2026Article
- Quantitative Assessment of Kidney Interstitial Fibrosis Using Optical Spectroscopy Mediated through a Fine Needle Probe.Kidney360 · 2026Article
- Multimodal Phasor Analysis for Digital Pathology: Quantitative Characterization of Liver Iron Overload.Chemical & biomedical imaging · 2026Article
- Antifibrotic therapy for Lower Urinary Tract Symptoms secondary to Benign Prostatic Hyperplasia.Prostate cancer and prostatic diseases · 2026Review
- A Novel Imaging Technique to Identify Vocal Fold Scar Formation.Laryngoscope investigative otolaryngology · 2026Article
- Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in crosslinking and post-gelation pH.Frontiers in bioengineering and biotechnology · 2026Article
- Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy.Journal of visualized experiments : JoVE · 2025Article
- Extracellular matrix repair and organization of chronic infected diabetic wounds treated with methacrylated chitosan-based hydrogels.Acta biomaterialia · 2025Article
- Quantitative Stain-Free Conjunctival Collagen Imaging in Cicatrizing Conjunctivitis Using Second Harmonic Generation-Two Photon Excitation Technology.Investigative ophthalmology & visual science · 2025Article
- Undergrowth Collagen Fibers Analysis by Fingerprint Enhancement Method.Biology of the cell · 2025Article
- Geometric characteristics of stromal collagen fibres in breast cancer using differential interference contrast microscopy.Journal of microscopy · 2025Article
- Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.Computational and structural biotechnology journal · 2025Article
- Prognostic and therapeutic implications of tumor-restrictive type III collagen in the breast cancer microenvironment.NPJ breast cancer · 2024Article
- Single-Cell Analysis of Bone-Marrow-Disseminated Tumour Cells.Diagnostics (Basel, Switzerland) · 2024Review
- Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice.American journal of physiology. Cell physiology · 2024Article
- Article
- Comparison of Retinal Metabolic Activity and Structural Development between rd10 Mice and Normal Mice Using Multiphoton Fluorescence Lifetime Imaging Microscopy.Current issues in molecular biology · 2024Article
- High-content stimulated Raman histology of human breast cancer.Theranostics · 2024Article
- Article
- Phasor identifier: A cloud-based analysis of phasor-FLIM data on Python notebooks.Biophysical reports · 2023Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
In this paper we have used second harmonic generation (SHG) and phasor approach to auto fluorescence lifetime imaging (FLIM) to obtain fingerprints of different collagens and then used these fingerprints to observe bone marrow fibrosis in the mouse femur. This is a label free approach towards fast automatable detection of fibrosis in tissue samples. FLIM has previously been used as a method of contrast in different tissues and in this paper phasor approach to FLIM is used to separate collagen I from collagen III, the markers of fibrosis, the largest groups of disorders that are often without any effective therapy. Often characterized by an increase in collagen content of the corresponding tissue, the samples are usually visualized by histochemical staining, which is pathologist dependent and cannot be automated.
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