ArticleNature chemistry2015
Organocatalytic removal of formaldehyde adducts from RNA and DNA bases.
Article in Nature chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- Spatially decoding genotype-associated epigenetic landscapes in human lymphoma FFPE tissues via epi-Patho-DBiT.Nature communications · 2026Article
- Evaluation and application of chemical decrosslinking in the context of histopathological spatial proteomics.bioRxiv : the preprint server for biology · 2026Article
- Expanding Epitranscriptomics to Non-Enzymatic RNA Modifications.Trends in chemistry · 2025Article
- An ancient influenza genome from Switzerland allows deeper insights into host adaptation during the 1918 flu pandemic in Europe.BMC biology · 2025Article
- Optimization of FFPE preparation and identification of gene attributes associated with RNA degradation.NAR genomics and bioinformatics · 2024Article
- Chemical RNA Cross-Linking: Mechanisms, Computational Analysis, and Biological Applications.JACS Au · 2023Review
- Nucleic acid adductomics - The next generation of adductomics towards assessing environmental health risks.The Science of the total environment · 2023Article
- Organocatalyst treatment improves variant calling and mutant detection in archival clinical samples.Scientific reports · 2022Article
- Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitment.Cell reports methods · 2022Article
- Case study: Targeted RNA-sequencing of aged formalin-fixed paraffin-embedded samples for understanding chemical mode of action.Toxicology reports · 2022Article
- Formation and repair of unavoidable, endogenous interstrand cross-links in cellular DNA.DNA repair · 2021Review
- Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems.Science (New York, N.Y.) · 2021Article
- Trapping Transient RNA Complexes by Chemically Reversible Acylation.Angewandte Chemie (International ed. in English) · 2020Article
- A Novel HPLC-Based Method to Investigate on RNA after Fixation.International journal of molecular sciences · 2020Article
- Exceptionally rapid oxime and hydrazone formation promoted by catalytic amine buffers with low toxicity.Chemical science · 2018Article
- NMR analyses on N-hydroxymethylated nucleobases - implications for formaldehyde toxicity and nucleic acid demethylases.Organic & biomolecular chemistry · 2018Article
- Demodifying RNA for Transcriptomic Analyses of Archival Formalin-Fixed Paraffin-Embedded Samples.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
- Cancer transcriptome profiling at the juncture of clinical translation.Nature reviews. Genetics · 2018Review
- On the rearrangement ofMonatshefte fur chemie · 2018Article
- On the Ability of Formaldehyde to Act as a Tethering Catalyst in Water.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2017Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Formaldehyde is universally used to fix tissue specimens, where it forms hemiaminal and aminal adducts with biomolecules, hindering the ability to retrieve molecular information. Common methods for removing these adducts involve extended heating, which can cause extensive degradation of nucleic acids, particularly RNA. Here, we show that water-soluble bifunctional catalysts (anthranilates and phosphanilates) speed the reversal of formaldehyde adducts of mononucleotides over standard buffers. Studies with formaldehyde-treated RNA oligonucleotides show that the catalysts enhance adduct removal, restoring unmodified RNA at 37 °C even when extensively modified, while avoiding the high temperatures that promote RNA degradation. Experiments with formalin-fixed, paraffin-embedded cell samples show that the catalysis is compatible with common RNA extraction protocols, with detectable RNA yields increased by 1.5-2.4-fold using a catalyst under optimized conditions and by 7-25-fold compared with a commercial kit. Such catalytic strategies show promise for general use in reversing formaldehyde adducts in clinical specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.