ArticlePloS one2015
Rapid, Precise, and Accurate Counts of Symbiodinium Cells Using the Guava Flow Cytometer, and a Comparison to Other Methods.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 73 citations in OpenAlex.
- Distinct transcriptomic strategies underlie differential heat tolerance in Symbiodiniaceae symbionts.The ISME journal · 2025Article
- Microbiome depletion and recovery in the sea anemone,mSystems · 2024Article
- Photosynthesis and other factors affecting the establishment and maintenance of cnidarian-dinoflagellate symbiosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Growth and Cell Size of MicroalgaMicroorganisms · 2024Article
- Comparative physiology reveals heat stress disrupts acid-base homeostasis independent of symbiotic state in the model cnidarian Exaiptasia diaphana.The Journal of experimental biology · 2024Article
- Rapid, high-throughput phenotypic profiling of endosymbiotic dinoflagellates (Symbiodiniaceae) using benchtop flow cytometry.PloS one · 2023Article
- Nutritional control regulates symbiont proliferation and life history in coral-dinoflagellate symbiosis.BMC biology · 2022Article
- Persistence of phenotypic responses to short-term heat stress in the tabletop coral Acropora hyacinthus.PloS one · 2022Article
- Flow cytometric monitoring of the bacterial phenotypic diversity in aquatic ecosystems.Journal of microbiology (Seoul, Korea) · 2021Article
- Widespread variation in heat tolerance and symbiont load are associated with growth tradeoffs in the coraleLife · 2021Article
- A review of coral bleaching specimen collection, preservation, and laboratory processing methods.PeerJ · 2021Article
- Insights into coral bleaching under heat stress from analysis of gene expression in a sea anemone model system.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations.Nature communications · 2020Article
- Experimental investigation of tidal and freshwater influence on Symbiodiniaceae abundance in Anthopleura elegantissima.PloS one · 2020Article
- High levels of floridoside at high salinity link osmoadaptation with bleaching susceptibility in the cnidarian-algal endosymbiosis.Biology open · 2019Article
- Cell Cytometry: Review and Perspective on Biotechnological Advances.Frontiers in bioengineering and biotechnology · 2019Review
- Glucose-Induced Trophic Shift in an Endosymbiont Dinoflagellate with Physiological and Molecular Consequences.Plant physiology · 2018Article
- Trace Metal Requirements and Interactions inFrontiers in microbiology · 2018Article
- High salinity conveys thermotolerance in the coral model Aiptasia.Biology open · 2017Article
- The genomics of recovery from coral bleaching.Proceedings. Biological sciences · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In studies of both the establishment and breakdown of cnidarian-dinoflagellate symbiosis, it is often necessary to determine the number of Symbiodinium cells relative to the quantity of host tissue. Ideally, the methods used should be rapid, precise, and accurate. In this study, we systematically evaluated methods for sample preparation and storage and the counting of algal cells using the hemocytometer, a custom image-analysis program for automated counting of the fluorescent algal cells, the Coulter Counter, or the Millipore Guava flow-cytometer. We found that although other methods may have value in particular applications, for most purposes, the Guava flow cytometer provided by far the best combination of precision, accuracy, and efficient use of investigator time (due to the instrument's automated sample handling), while also allowing counts of algal numbers over a wide range and in small volumes of tissue homogenate. We also found that either of two assays of total homogenate protein provided a precise and seemingly accurate basis for normalization of algal counts to the total amount of holobiont tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.