Evidence map›Paper›PMID 26291078›Full record

ArticlePloS one2015

Natural Killer p46 Controls Hepatitis B Virus Replication and Modulates Liver Inflammation.

Wanyu Li, Yanfang Jiang, Xiaomei Wang, Jinglan Jin, Yue Qi, Xiumei Chi, Hong Zhang, Xiangwei Feng, Junqi Niu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. The antifibrotic role of natural killer cells in liver fibrosis.Experimental biology and medicine (Maywood, N.J.) · 2022
    Review
  6. Review
  7. Review
  8. Article
  9. Article
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  12. Article
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  14. Association betweenInternational journal of organ transplantation medicine · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Wanyu LiDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Yanfang JiangDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Xiaomei WangDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Jinglan JinDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Yue QiDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Xiumei ChiDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Hong ZhangDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Xiangwei FengDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Junqi NiuDepartment of Hepatology, First Hospital, Jilin University, No. 71 Xinmin Street, Changchun, 130021, China.
Jilin University · CNFirst Hospital of Jilin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Natural killer (NK) cells play an important role in hepatitis B virus (HBV) infection control, and are regulated by a complex network of activating and inhibitory receptors. However, NK cell activity in HBV patients remains poorly understood. The objective of this study was to investigate the phenotypic and functional characteristics of circulating NK cells in patients during different chronic hepatitis B (CHB) infection stages. We investigated NK cell phenotypes, receptor expression and function in 86 CHB patients and 20 healthy controls. NK cells were purified and NK cell subsets were characterized by flow cytometry. Cytotoxic activity (CD107a) and interferon-gamma (IFN-γ) secretion were examined, and Natural Killer p46 (NKP46) blockade and spontaneous NK cell cytolytic activity against K562, HepG2 and HepG2.215 cell lines was studied. Activating NKp46 receptor expression was higher in inactive HBsAg carriers when compared with other groups (p = 0.008). NKp46 expression negatively correlated with HBV DNA (R = -0.253, p = 0.049) and ALT (R = -0.256, p = 0.045) levels. CD107a was higher in immune-activated groups when compared with immune-tolerant groups (p = 0.039). CD107a expression was related to viral load (p = 0.02) and HBeAg status (p = 0.024). In vitro NKp46 blockade reduced NK cell cytolytic activity against HepG2 and HepG2.215 cell lines (p = 0.02; p = 0.039). Furthermore, NK cells from high viral load CHB patients displayed significantly lower specific cytolytic activity against anti-NKp46-loaded K562 targets (p = 0.0321). No significant differences were observed in IFN-γ secretion (p > 0.05). In conclusion, NKp46 expression regulates NK cell cytolytic function. NKp46 may moderate NK cell activity during HBV replication suppression and HBV-associated liver damage and may be critical for NK cell activity during CHB infection.

Indexed as

AdultCase-Control StudiesCell Line, TumorDNA, ViralFemaleHepatitis B, ChronicHepatitis B e AntigensHepatitis B virusHep G2 CellsHumansInflammationInterferon-gammaK562 CellsKiller Cells, NaturalLiverMaleDNA, ViralHepatitis B e AntigensInterferon-gammaNatural Cytotoxicity Triggering Receptor 1

Identifiers

PMID26291078
PMCPMC4546267
OpenAlexW1181515844

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.