ArticlePeerJ2015
To beat or not to beat a tick: comparison of DNA extraction methods for ticks (Ixodes scapularis).
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 64 citations in OpenAlex.
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- A framework for optimising arthropod DNA quality and quantity for modern sequencing tools using hard ticks (Ixodidae).Parasitology research · 2026Article
- Sexual and regional differences in the microbiome and functional metagenome of the lone star tick, Amblyomma americanum.Animal microbiome · 2025Article
- The diversity of sandflies (Psychodidae: Phlebotominae) and the presence of Leishmania spp. DNA in potential vectors of the Mbaracayú Forest Biosphere Reserve, Canindeyú, Paraguay: New records and findings.PLoS neglected tropical diseases · 2025Article
- Genome-wide insights into genetic diversity of endemic and non-endemic Ixodes ricinus populations.Scientific reports · 2025Article
- Host-Specificity and Network Structure of Tick Microbiota in Co-Distributed Species From the Iberian Peninsula.Ecology and evolution · 2025Article
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- Comparison of DNA extraction methods on different sample matrices within the same terrestrial ecosystem.Scientific reports · 2024Article
- Sanitation in urban areas may limit the spread of antimicrobial resistance via flies.PloS one · 2024Article
- Update on tick-borne pathogens detection methods within ticks.Current research in parasitology & vector-borne diseases · 2024Article
- Detection of multiple tick-borne pathogens inCurrent research in parasitology & vector-borne diseases · 2023Article
- Comparison of DNA Extraction and Amplification Techniques for Use with Engorged Hard-Bodied Ticks.Microorganisms · 2022Article
- Changes in theFrontiers in microbiology · 2022Article
- First report regarding molecular epidemiology and novel variant identification ofSaudi journal of biological sciences · 2021Article
- The tick fauna in Istanbul, Turkey, from 2013 to 2017 and identification of their pathogens by multiplex PCR: an epidemiological study.Experimental & applied acarology · 2021Article
- Molecular epidemiology of Theileria annulata infection of cattle in Layyah District, Pakistan.Experimental & applied acarology · 2021Article
- A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan).Current microbiology · 2021Article
- A Beginner's Guide to Collecting Questing Hard Ticks (Acari: Ixodidae): A Standardized Tick Dragging Protocol.Journal of insect science (Online) · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background. Blacklegged ticks (Ixodes scapularis) are important disease vectors in the United States, known to transmit a variety of pathogens to humans, including bacteria, protozoa, and viruses. Their importance as a disease vector necessitates reliable and comparable methods for extracting microbial DNA from ticks. Furthermore, to explore the population genetics or genomics of this tick, appropriate DNA extraction techniques are needed for both the vector and its microbes. Although a few studies have investigated different methods of DNA isolation from ticks, they are limited in the number and types of DNA extraction and lack species-specific quantification of DNA yield. Methods. Here we determined the most efficient and consistent method of DNA extraction from two different developmental stages of I. scapularis-nymph and adult-that are the most important for disease transmission. We used various methods of physical disruption of the hard, chitinous exoskeleton, as well as commercial and non-commercial DNA isolation kits. To gauge the effectiveness of these methods, we quantified the DNA yield and confirmed the DNA quality via PCR of both tick and microbial genetic material. Results. DNA extraction using the Thermo GeneJET Genomic DNA Purification Kit resulted in the highest DNA yields and the most consistent PCR amplification when combined with either cutting or bead beating with select matrices across life stages. DNA isolation methods using ammonium hydroxide as well as the MoBio PowerSoil kit also produced strong and successful PCR amplification, but only for females. Discussion. We contrasted a variety of readily available methods of DNA extraction from single individual blacklegged ticks and presented the results through a quantitative and qualitative assessment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.