ArticlePeerJ2015
Comprehensive analyses of genomes, transcriptomes and metabolites of neem tree.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Comparative genomics provides insights into the biogeographic and biochemical diversity of meliaceous species.Nature communications · 2025Article
- Comprehensive genome-wide analysis of theFrontiers in plant science · 2025Article
- Meliaceae genomes provide insights into wood development and limonoids biosynthesis.Plant biotechnology journal · 2023Article
- Deciphering the key pathway for triterpenoid biosynthesis inFrontiers in plant science · 2023Review
- Genomic Analysis Based on Chromosome-Level Genome Assembly Reveals an Expansion of Terpene Biosynthesis ofFrontiers in plant science · 2022Article
- De Novo Assembly and Species-Specific Marker Development as a Useful Tool for the Identification ofCurrent issues in molecular biology · 2021Article
- A complete sequence of mitochondrial genome of Neolamarckia cadamba and its use for systematic analysis.Scientific reports · 2021Article
- Development, characterization, functional annotation and validation of genomic and genic-SSR markers using de novo next generation sequencing in3 Biotech · 2021Article
- Characterization of microRNAs from neem (3 Biotech · 2021Article
- Azadirachta indica MicroRNAs: Genome-Wide Identification, Target Transcript Prediction, and Expression Analyses.Applied biochemistry and biotechnology · 2021Article
- Identification of key enzymes responsible for protolimonoid biosynthesis in plants: Opening the door to azadirachtin production.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Genomic resources for the Neotropical tree genus Cedrela (Meliaceae) and its relatives.BMC genomics · 2019Article
- Mining and Development of Novel SSR Markers Using Next Generation Sequencing (NGS) Data in Plants.Molecules (Basel, Switzerland) · 2018Review
- De novo transcriptome sequencing and SSR markers development for Cedrela balansae C.DC., a native tree species of northwest Argentina.PloS one · 2018Article
- Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids.Scientific reports · 2017Article
- Induction of Autophagy and Apoptosis via PI3K/AKT/TOR Pathways by Azadirachtin A in Spodoptera litura Cells.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neem (Azadirachta indica A. Juss) is one of the most versatile tropical evergreen tree species known in India since the Vedic period (1500 BC-600 BC). Neem tree is a rich source of limonoids, having a wide spectrum of activity against insect pests and microbial pathogens. Complex tetranortriterpenoids such as azadirachtin, salanin and nimbin are the major active principles isolated from neem seed. Absolutely nothing is known about the biochemical pathways of these metabolites in neem tree. To identify genes and pathways in neem, we sequenced neem genomes and transcriptomes using next generation sequencing technologies. Assembly of Illumina and 454 sequencing reads resulted in 267 Mb, which accounts for 70% of estimated size of neem genome. We predicted 44,495 genes in the neem genome, of which 32,278 genes were expressed in neem tissues. Neem genome consists about 32.5% (87 Mb) of repetitive DNA elements. Neem tree is phylogenetically related to citrus, Citrus sinensis. Comparative analysis anchored 62% (161 Mb) of assembled neem genomic contigs onto citrus chromomes. Ultrahigh performance liquid chromatography-mass spectrometry-selected reaction monitoring (UHPLC-MS/SRM) method was used to quantify azadirachtin, nimbin, and salanin from neem tissues. Weighted Correlation Network Analysis (WCGNA) of expressed genes and metabolites resulted in identification of possible candidate genes involved in azadirachtin biosynthesis pathway. This study provides genomic, transcriptomic and quantity of top three neem metabolites resource, which will accelerate basic research in neem to understand biochemical pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.