ArticleJournal of animal science and technology2015
In vitro maturation of ovine oocyte in a modified granulosa cells co-culture system and alpha-tocopherol supplementation: effects on nuclear maturation and cleavage.
Article in Journal of animal science and technology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Artificial activation of clinically failed human oocytes enables euploid blastocyst formation.Journal of assisted reproduction and genetics · 2026Article
- Comparative effects of insulin-like growth factor-1, lycopene, and α-tocopherol on mitochondrial dynamics and developmental competence of buffalo (Veterinary world · 2026Article
- Granulosa Cells Improved Mare Oocyte Cytoplasmic Maturation by Providing Collagens.Frontiers in cell and developmental biology · 2022Article
- Effect of Ethanol on Parthenogenetic Activation and α-Tocopherol Supplementation duringCurrent issues in molecular biology · 2021Article
- The reaggregation of normal granulosa-cumulus cells and mouse oocytes with polycystic ovarian syndrome in vitro: An experimental study.International journal of reproductive biomedicine · 2021Article
- Lnc-GULP1-2:1 affects granulosa cell proliferation by regulating COL3A1 expression and localization.Journal of ovarian research · 2021Article
- A Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures.International journal of molecular sciences · 2018Article
- Article
- Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress.PloS one · 2017Article
- The Impact of Endometriosis on Intracellular Calcium Levels, Cyclic Dependent Kinase 1 (Cdk1) Expression, and Cyclin B Expression in Post-Ovulation Oocytes of Mice Model.Journal of reproduction & infertilityArticle
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5 authors.
Funding
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Abstract
This study was designed to investigate the effects of α-tocopherol and granulosa cells monolayer on nuclear maturation and cleavage rates of ovine cumulus-oocyte complexes (COCs). The COCs (n = 2814) were matured in maturation medium supplemented with various concentration of α-tocopherol (0, 5, 10, 15 μg/ml), oocytes were incubated at 39 °C with 5 % CO2 for 24 h in three culture systems: (a) maturation medium (MM; n = 884), (b) co-cultured with granulosa cells (CG; n = 982) and (c) co-cultured with granulosa cells and cells were further cultured in MM for 12 h (CG + 12hMM; n = 948). Our results showed that α-tocopherol had no effect on GVBD and MII as compared to control group, but when α-tocopherol added to maturation medium the rate of cleavage decreased. This indicates interaction of above mentioned factors in any of the treatments showed no significant differences on the rate of maturation and cleavage stages (MII, GVBD and cleavage) (p > 0.05). The oocytes co-cultured with granulosa cells for 24 h had beneficial effects on cleavage rate. The maximum MII and cleavage rates were achieved when oocytes had extra 12 h culture in the maturation medium without granulosa cells. Results also showed our modified co-culture system (CG + 12hMM), improved rates of MII and the cleavage in comparison with other studied maturation systems.
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