Evidence map›Paper›PMID 26290581›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2015

E-cadherin junction formation involves an active kinetic nucleation process.

Kabir H Biswas, Kevin L Hartman, Cheng-han Yu, Oliver J Harrison, Hang Song, Adam W Smith, William Y C Huang, Wan-Chen Lin, Zhenhuan Guo, Anup Padmanabhan and 6 more

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 101 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Characterization of early and late events of adherens junction assembly.bioRxiv : the preprint server for biology · 2024
    Article
  8. Review
  9. BRET-based biosensors for SARS-CoV-2 oligonucleotide detection.Frontiers in bioengineering and biotechnology · 2024
    Article
  10. Building Biomaterials to Mimic 3D Cell-Cell Junctions.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  11. Article
  12. Article
  13. Mechanics of cell-cell junctions.Biophysical journal · 2023
    Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 7 institutions in 3 countries.

Kabir H BiswasMechanobiology Institute, National University of Singapore, Singapore 117411;
Kevin L HartmanMechanobiology Institute, National University of Singapore, Singapore 117411; Department of Chemistry, University of California, Berkeley, CA 94720;
Cheng-han YuMechanobiology Institute, National University of Singapore, Singapore 117411;
Oliver J HarrisonDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Howard Hughes Medical Institute, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032;
Hang SongDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Howard Hughes Medical Institute, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032;
Adam W SmithHoward Hughes Medical Institute, University of California, Berkeley, CA 94720;
William Y C HuangDepartment of Chemistry, University of California, Berkeley, CA 94720;
Wan-Chen LinHoward Hughes Medical Institute, University of California, Berkeley, CA 94720;
Zhenhuan GuoMechanobiology Institute, National University of Singapore, Singapore 117411;
Anup PadmanabhanMechanobiology Institute, National University of Singapore, Singapore 117411;
Sergey M TroyanovskyDepartment of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611;
Michael L DustinKennedy Institute, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Headington OX3 7FY, United Kingdom;
Lawrence ShapiroDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032;
Barry HonigDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032; Howard Hughes Medical Institute, Columbia University, New York, NY 10032; Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032; bh6@cumc.columbia.edu biezbr@nus.edu.sg jtgroves@lbl.gov.
Ronen Zaidel-BarMechanobiology Institute, National University of Singapore, Singapore 117411; Department of Biomedical Engineering, National University of Singapore, Singapore 117411; bh6@cumc.columbia.edu biezbr@nus.edu.sg jtgroves@lbl.gov.
Jay T GrovesMechanobiology Institute, National University of Singapore, Singapore 117411; Department of Chemistry, University of California, Berkeley, CA 94720; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720; Physical Biosciences and Materials Sciences Divisions, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720 bh6@cumc.columbia.edu biezbr@nus.edu.sg jtgroves@lbl.gov.
National University of Singapore · SGUniversity of California, Berkeley · USHoward Hughes Medical Institute · USColumbia University · USLawrence Berkeley National Laboratory · USNorthwestern University · USUniversity of Oxford · GB

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Daniela E Matei · 1993 to 2026
$153.9M
Inter-junctional signaling in epithelial junctional complexR01AR044016 · NIAMS · WASHINGTON UNIVERSITY · PI TROYANOVSKY, SERGEY M · 1996 to 2020
$6.8M
Requirement for Sensitive T Cell Response to AntigenR37AI043542 · NIAID · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI DUSTIN, MICHAEL LORAN · 2007 to 2016
$3.6M
MOLECULAR BASIS OF CADHERIN MEDIATED CELL ADHESIONR01GM062270 · NIGMS · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI SHAPIRO, LAWRENCE S · 2001 to 2015
$3.4M
REQUIREMENT FOR SENSITIVE T CELL RESPONSE TO ANTIGENR01AI043542 · NIAID · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI DUSTIN, MICHAEL L · 1999 to 2005
$1.5M
Inter-junctional signaling in epithelial junctional complexR56AR044016 · NIAMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI TROYANOVSKY, SERGEY M · 2015 to 2015
$435k
Howard Hughes Medical InstituteNCI NIH HHS P30 CA060553NIAID NIH HHS AI043542NIAID NIH HHS R01 AI043542NIAID NIH HHS R37 AI043542NIAMS NIH HHS R01 AR044016NIAMS NIH HHS R56 AR044016NIGMS NIH HHS R01 GM062270Wellcome Trust 100262Wellcome Trust 100262/Z/12/Z
6 · The paper itself

Abstract

Epithelial (E)-cadherin-mediated cell-cell junctions play important roles in the development and maintenance of tissue structure in multicellular organisms. E-cadherin adhesion is thus a key element of the cellular microenvironment that provides both mechanical and biochemical signaling inputs. Here, we report in vitro reconstitution of junction-like structures between native E-cadherin in living cells and the extracellular domain of E-cadherin (E-cad-ECD) in a supported membrane. Junction formation in this hybrid live cell-supported membrane configuration requires both active processes within the living cell and a supported membrane with low E-cad-ECD mobility. The hybrid junctions recruit α-catenin and exhibit remodeled cortical actin. Observations suggest that the initial stages of junction formation in this hybrid system depend on the trans but not the cis interactions between E-cadherin molecules, and proceed via a nucleation process in which protrusion and retraction of filopodia play a key role.

Indexed as

Intercellular JunctionsBiophysicsCadherinsCell LineCytoskeletonHumansKineticsLipid BilayersSignal TransductionCadherinsLipid Bilayersadhesionbilayercadherindiffusionnucleation

Identifiers

PMID26290581
PMCPMC4568248
OpenAlexW1935095490

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.