ArticleThe Plant cell2015
Small RNA Derived from the Virulence Modulating Region of the Potato spindle tuber viroid Silences callose synthase Genes of Tomato Plants.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 125 citations in OpenAlex.
- MicroRNA-mediated regulation of cell wall dynamics and intercellular communication under stress.Frontiers in plant science · 2026Review
- RNA Interference in Plant Interactions with Pathogenic Microorganisms: A Weapon or a Liability?Current issues in molecular biology · 2025Review
- Genome-Wide Analysis of Callose Synthase (CALS) Genes in Cabbage (International journal of molecular sciences · 2025Article
- Small RNAs derived from avocado sunblotch viroid and their association with bleaching symptoms: implications for pathogenesis in avocado sunblotch disease.Archives of virology · 2025Article
- Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication.Plants (Basel, Switzerland) · 2024Review
- Profiling of small RNAs derived from tomato brown rugose fruit virus in infectedFrontiers in microbiology · 2024Article
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- Roles of RNA silencing in viral and non-viral plant immunity and in the crosstalk between disease resistance systems.Nature reviews. Molecular cell biology · 2022Review
- Transcriptomics Advancement in the Complex Response of Plants to Viroid Infection.International journal of molecular sciences · 2022Review
- Article
- Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis.Genome biology · 2021Article
- Transcriptome Analysis Reveals Dynamic Cultivar-Dependent Patterns of Gene Expression in Potato Spindle Tuber Viroid-Infected Pepper.Plants (Basel, Switzerland) · 2021Article
- Article
- Article
- Symptom Severity, Infection Progression and Plant Responses in Solanum Plants Caused by Three Pospiviroids Vary with the Inoculation Procedure.International journal of molecular sciences · 2021Article
- Review
- Degradome Analysis of Tomato andInternational journal of molecular sciences · 2021Article
- Variability, Functions and Interactions of Plant Virus Movement Proteins: What Do We Know So Far?Microorganisms · 2021Review
- Tolerance Even to Lethal Strain of Potato Spindle Tuber Viroid Found in Wild Tomato Species Can Be Introduced by Crossing.Plants (Basel, Switzerland) · 2021Article
- An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids.International journal of molecular sciences · 2021Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tomato (Solanum lycopersicum) callose synthase genes CalS11-like and CalS12-like encode proteins that are essential for the formation of callose, a major component of pollen mother cell walls; these enzymes also function in callose formation during pathogen infection. This article describes the targeting of these callose synthase mRNAs by a small RNA derived from the virulence modulating region of two Potato spindle tuber viroid variants. More specifically, viroid infection of tomato plants resulted in the suppression of the target mRNAs up to 1.5-fold, depending on the viroid variant used and the gene targeted. The targeting of these mRNAs by RNA silencing was validated by artificial microRNA experiments in a transient expression system and by RNA ligase-mediated rapid amplification of cDNA ends. Viroid mutants incapable of targeting callose synthase mRNAs failed to induce typical infection phenotypes, whereas a chimeric viroid obtained by swapping the virulence modulating regions of a mild and a severe variant of Potato spindle tuber viroid greatly affected the accumulation of viroids and the severity of disease symptoms. These data provide evidence of the silencing of multiple genes by a single small RNA derived from a viroid.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.