ArticleScientific reports2015
Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Theory for High-Throughput Genetic Interaction Screening.ACS synthetic biology · 2023Article
- Specific detection of tau seeding activity in Alzheimer's disease using rationally designed biosensor cells.Molecular neurodegeneration · 2023Article
- Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activation.Molecular neurodegeneration · 2023Article
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- What do we know about dynamic glucose-enhanced (DGE) MRI and how close is it to the clinics? Horizon 2020 GLINT consortium report.Magma (New York, N.Y.) · 2022Review
- Continuous Monitoring of Tau-Induced Neurotoxicity in Patient-Derived iPSC-Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.Nature cell biology · 2021Article
- Visualization and Manipulation of Intracellular Signaling.Advances in experimental medicine and biology · 2021Review
- Shedding new light on RhoA signalling as a drug targetSmall GTPases · 2020Article
- An AND-Gated Drug and Photoactivatable Cre-ACS synthetic biology · 2019Article
- Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Live-Cell FRET Imaging Reveals a Role of Extracellular Signal-Regulated Kinase Activity Dynamics in Thymocyte Motility.iScience · 2018Article
- A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging.Scientific reports · 2018Article
- Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensors.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Future Perspective of Single-Molecule FRET Biosensors and Intravital FRET Microscopy.Biophysical journal · 2016Review
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biosensors based on the principle of Förster (or fluorescence) resonance energy transfer (FRET) have been developed to visualize spatio-temporal dynamics of signalling molecules in living cells. Many of them adopt a backbone of intramolecular FRET biosensor with a cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) as donor and acceptor, respectively. However, there remains the difficulty of establishing cells stably expressing FRET biosensors with a YFP and CFP pair by lentiviral or retroviral gene transfer, due to the high incidence of recombination between YFP and CFP genes. To address this, we examined the effects of codon-diversification of YFP on the recombination of FRET biosensors introduced by lentivirus or retrovirus. The YFP gene that was fully codon-optimized to E.coli evaded the recombination in lentiviral or retroviral gene transfer, but the partially codon-diversified YFP did not. Further, the length of spacer between YFP and CFP genes clearly affected recombination efficiency, suggesting that the intramolecular template switching occurred in the reverse-transcription process. The simple mathematical model reproduced the experimental data sufficiently, yielding a recombination rate of 0.002-0.005 per base. Together, these results show that the codon-diversified YFP is a useful tool for expressing FRET biosensors by lentiviral or retroviral gene transfer.
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