ArticleBMC musculoskeletal disorders2015
Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing.
Article in BMC musculoskeletal disorders, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- The Functions and Mechanisms of Tendon Stem/Progenitor Cells in Tendon Healing.Stem cells international · 2023Review
- Cell autonomous TGFβ signaling is essential for stem/progenitor cell recruitment into degenerative tendons.Stem cell reports · 2021Article
- Complete mid-portion rupture of the rat achilles tendon leads to remote and time-mismatched changes in uninjured regions.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2021Article
- Zonal characterization and differential trilineage potentials of equine intrasynovial deep digital flexor tendon-derived cells.BMC veterinary research · 2021Article
- Tendon Stem/Progenitor Cell Subpopulations and Their Implications in Tendon Biology.Frontiers in cell and developmental biology · 2021Article
- Article
- PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells.Stem cell research & therapy · 2019Article
- Mechanical, histological, and functional properties remain inferior in conservatively treated Achilles tendons in rodents: Long term evaluation.Journal of biomechanics · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe recent discovery of residing tendon stem/progenitor cells has triggered a growing interest in stem cells as a useful tool in tendon repair. Our knowledge of their involvement in naturally healing tendons is, however, sparse. The aim of this study was to identify and determine stem/progenitor cells in relation to different healing phases and regions in a rat model of Achilles tendon rupture.
methodsSurgery was performed to create a mid-tendon rupture on the right Achilles tendon of 24 rats, whereas the left tendon was used as a control. Tendons were harvested at one, two, eight and 17 weeks post-rupture and stained with antibodies specific to stem/progenitor cells (Octamer-binding transcription factor 3/4 (Oct 3/4) and nucleostemin), migrating cells (Dynamin 2 (Dyn 2)) and leukocytes (CD45). A histological examination was performed on sections stained with Alcian blue.
resultsAt one and two weeks post-rupture, a large number of stem/progenitor cells were discovered throughout the tendon. Most of these cells were nucleostemin positive, whereas only a few Oct 3/4-positive cells were found, mainly situated inside the injury region (I region). At eight and 17 weeks, the increment in stem/progenitor cells had diminished to equal that in the control tendons. At all time points, Oct 3/4-positive cells were also found in the connective tissue surrounding the tendon and at the muscle-tendon junction in both ruptured and control tendons and were often seen at the same location as the migration marker, Dyn 2.
conclusionsThe whole length of the Achilles tendon is infiltrated by stem/progenitor cells at early time points after a mid-tendon rupture. However, different stem/progenitor cell populations exhibit varying anatomical and temporal expressions during Achilles tendon healing, suggesting distinct reparative implications. Oct 3/4 may thus act as a more local, migrating stem/progenitor cell involved in injury-site-specific regenerative effects, as compared to the more general proliferative role of nucleostemin-positive stem/progenitor cells.
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