Evidence map›Paper›PMID 26290268›Full record

ArticleNeurochemical research2016

Molecular Changes in Sub-lesional Muscle Following Acute Phase of Spinal Cord Injury.

Nakul P Thakore, Supriti Samantaray, Sookyoung Park, Kenkichi Nozaki, Joshua A Smith, April Cox, James Krause, Naren L Banik

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurochemical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Mitochondria Initiate and Regulate Sarcopenia.Exercise and sport sciences reviews · 2017
    Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nakul P ThakoreDepartment of Neurosurgery and Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, 309 CSB, MSC 606, Charleston, SC, 29425, USA.
Supriti SamantarayDepartment of Neurosurgery and Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, 309 CSB, MSC 606, Charleston, SC, 29425, USA.
Sookyoung ParkDepartment of Neurosurgery and Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, 309 CSB, MSC 606, Charleston, SC, 29425, USA.
Kenkichi NozakiDepartment of Neurosurgery and Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, 309 CSB, MSC 606, Charleston, SC, 29425, USA.
Joshua A SmithDepartment of Neurosurgery and Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, 309 CSB, MSC 606, Charleston, SC, 29425, USA.
April CoxDepartment of Neurosurgery and Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, 309 CSB, MSC 606, Charleston, SC, 29425, USA.
James KrauseDepartment of Health Sciences and Research, College of Health Professions, Medical University of South Carolina, Charleston, SC, USA.
Naren L BanikDepartment of Neurosurgery and Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, 309 CSB, MSC 606, Charleston, SC, 29425, USA. baniknl@musc.edu.

Funding

ROLE OF PROTEINASE IN SPINAL CORD INJURYR01NS031622 · NINDS · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI BANIK, NAREN L · 1994 to 2011
$3.4M
MUSC FACIL EXPANSION &RENOV: SLE C06RR015455 · NCRR · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI RAYMOND, JOHN R · 2002 to 2002
$2.0M
BLRD VA I01 BX001262BLRD VA I01 BX002349NCRR NIH HHS C06 RR015455NINDS NIH HHS NS031622NINDS NIH HHS R01 NS031622
6 · The paper itself

Abstract

To clarify the molecular changes of sublesional muscle in the acute phase of spinal cord injury (SCI), a moderately severe injury (40 g cm) was induced in the spinal cord (T10 vertebral level) of adult male Sprague-Dawley rats (injury) and compared with sham (laminectomy only). Rats were sacrificed at 48 h (acute) post injury, and gastrocnemius muscles were excised. Morphological examination revealed no significant changes in the muscle fiber diameter between the sham and injury rats. Western blot analyses performed on the visibly red, central portion of the gastrocnemius muscle showed significantly higher expression of muscle specific E3 ubiquitin ligases (muscle ring finger-1 and muscle atrophy f-box) and significantly lower expression of phosphorylated Akt-1/2/3 in the injury group compared to the sham group. Cyclooxygenase 2, tumor necrosis factor alpha (TNF-α), and caspase-1, also had a significantly higher expression in the injury group; although, the mRNA levels of TNF-α and IL-6 did not show any significant difference between the sham and injury groups. These results suggest activation of protein degradation, deactivation of protein synthesis, and development of inflammatory reaction occurring in the sublesional muscles in the acute phase of SCI before overt muscle atrophy is seen.

Indexed as

Gene Expression ProfilingAnimalsCytokinesInflammation MediatorsMaleMuscle, SkeletalRatsRats, Sprague-DawleySpinal Cord InjuriesCytokinesInflammation MediatorsE3-ubiquitin ligasesInflammationMuscle atrophyProtein degradationProtein synthesisSpinal cord injury

Identifiers

PMID26290268
PMCPMC9727651

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.