ArticleRNA (New York, N.Y.)2015
Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- DNA Nanomachine (DNM) Biplex Assay for DifferentiatingInternational journal of molecular sciences · 2023Article
- Single-tube isothermal label-free fluorescent sensor for pathogen detection based on genetic signatures.Frontiers in chemistry · 2022Article
- Interrogation of highly structured RNA with multicomponent deoxyribozyme probes at ambient temperatures.RNA (New York, N.Y.) · 2020Article
- Cascade of deoxyribozymes for the colorimetric analysis of drug resistance in Mycobacterium tuberculosis.Biosensors & bioelectronics · 2020Article
- Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.Sensors and actuators. B, Chemical · 2019Article
- Species Typing of Nontuberculous Mycobacteria by Use of Deoxyribozyme Sensors.Clinical chemistry · 2019Article
- FaptaSyme: A Strategy for Converting a Monomer/Oligomer-Nonselective Aptameric Sensor into an Oligomer-Selective One.Chembiochem : a European journal of chemical biology · 2018Article
- A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids.Chemical communications (Cambridge, England) · 2017Article
- Multiplex detection of extensively drug resistant tuberculosis using binary deoxyribozyme sensors.Biosensors & bioelectronics · 2017Article
- Divide and Control: Comparison of Split and Switch Hybridization Sensors.ChemistrySelect · 2017Article
- DNA nanotechnology for nucleic acid analysis: multifunctional molecular DNA machine for RNA detection.Chemical communications (Cambridge, England) · 2016Article
- DNA Antenna Tile-Associated Deoxyribozyme Sensor with Improved Sensitivity.Chembiochem : a European journal of chemical biology · 2016Article
- Time course of large ribosomal subunit assembly in E. coli cells overexpressing a helicase inactive DbpA protein.RNA (New York, N.Y.) · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Mutations in ribosomal RNA (rRNA) have traditionally been detected by the primer extension assay, which is a tedious and multistage procedure. Here, we describe a simple and straightforward fluorescence assay based on binary deoxyribozyme (BiDz) sensors. The assay uses two short DNA oligonucleotides that hybridize specifically to adjacent fragments of rRNA, one of which contains a mutation site. This hybridization results in the formation of a deoxyribozyme catalytic core that produces the fluorescent signal and amplifies it due to multiple rounds of catalytic action. This assay enables us to expedite semi-quantification of mutant rRNA content in cell cultures starting from whole cells, which provides information useful for optimization of culture preparation prior to ribosome isolation. The method requires less than a microliter of a standard Escherichia coli cell culture and decreases analysis time from several days (for primer extension assay) to 1.5 h with hands-on time of ∼10 min. It is sensitive to single-nucleotide mutations. The new assay simplifies the preliminary analysis of RNA samples and cells in molecular biology and cloning experiments and is promising in other applications where fast detection/quantification of specific RNA is required.
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