ArticleScientific reports2015
The Multiple DSF-family QS Signals are Synthesized from Carbohydrate and Branched-chain Amino Acids via the FAS Elongation Cycle.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 91 citations in OpenAlex.
- The RpfF Acyl-ACP thioesterase of major rice pathogen Xanthomonas oryzae pv. oryzae is essential for maintenance of membrane function and virulence.PLoS pathogens · 2026Article
- A global stress alarmone couples nutrient limitation to suppression of social signaling in bacteria.iScience · 2026Article
- Review
- The phytohormone indole-3-acetic acid induces quorum sensing signal DSF turnover via a positive feedback biosynthetic loop in the phytopathogen Xanthomonas campestris.Stress biology · 2026Article
- Functional disparities of malonyl-ACP decarboxylase betweenApplied and environmental microbiology · 2025Article
- Diffusible Signal Factors andBiology · 2025Review
- An atypical 3-ketoacyl ACP synthase III required for acyl homoserine lactone synthesis inApplied and environmental microbiology · 2024Article
- Research on Diffusible Signal Factor-Mediated Quorum Sensing inMolecules (Basel, Switzerland) · 2023Review
- Microbe Related Chemical Signalling and Its Application in Agriculture.International journal of molecular sciences · 2022Review
- Bacterial Quorum-Sensing Signal DSF Inhibits LPS-Induced Inflammations by Suppressing Toll-like Receptor Signaling and Preventing Lysosome-Mediated Apoptosis in Zebrafish.International journal of molecular sciences · 2022Article
- Research Progress and Hopeful Strategies of Application of Quorum Sensing in Food, Agriculture and Nanomedicine.Microorganisms · 2022Article
- BDSF Is a Degradation-Prone Quorum-Sensing Signal Detected by the Histidine Kinase RpfC ofApplied and environmental microbiology · 2022Article
- Article
- The diffusible signal factor synthase, RpfF, in Xanthomonas oryzae pv. oryzae is required for the maintenance of membrane integrity and virulence.Molecular plant pathology · 2022Article
- Article
- Review
- Review
- Bacterial Quorum-Sensing Systems and Their Role in Intestinal Bacteria-Host Crosstalk.Frontiers in microbiology · 2021Review
- Comparative Proteomics ofMicroorganisms · 2020Article
- The bacterial quorum sensing signal DSF hijacksLife science alliance · 2020Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the diffusible signal factor (DSF) family are a novel class of quorum sensing (QS) signals in diverse Gram-negative bacteria. Although previous studies have identified RpfF as a key enzyme for the biosynthesis of DSF family signals, many questions in their biosynthesis remain to be addressed. In this study with the phytopathogen Xanthomonas campestris pv. campestris (Xcc), we show that Xcc produces four DSF-family signals (DSF, BDSF, CDSF and IDSF) during cell culture, and that IDSF is a new functional signal characterized as cis-10-methyl-2-dodecenoic acid. Using a range of defined media, we further demonstrate that Xcc mainly produces BDSF in the presence of carbohydrates; leucine and valine are the primary precursor for DSF biosynthesis; isoleucine is the primary precursor for IDSF biosynthesis. Furthermore, our biochemical analyses show that the key DSF synthase RpfF has both thioesterase and dehydratase activities, and uses 3-hydroxydedecanoyl-ACP as a substrate to produce BDSF. Finally, our results show that the classic fatty acid synthesis elongation cycle is required for the biosynthesis of DSF-family signals. Taken all together, these findings establish a general biosynthetic pathway for the DSF-family quorum sensing signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.