ArticleInternational journal for parasitology. Drugs and drug resistance2015
Viability of developmental stages of Schistosoma mansoni quantified with xCELLigence worm real-time motility assay (xWORM).
Article in International journal for parasitology. Drugs and drug resistance, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- The Anthelmintic Activity of Stonefish (Toxins · 2025Article
- In vitro culture and confocal microscopy study of Maritrema gratiosum Nicoll, 1907 (Digenea): From metacercaria to ovigerous adult.Parasitology research · 2025Article
- A phenotypic screen of the Global Health Priority Box identifies an insecticide with anthelmintic activity.Parasites & vectors · 2024Article
- Proteomic differences between extracellular vesicles and extracellular vesicle-depleted excretory/secretory products of barber's pole worm.Parasites & vectors · 2024Article
- Article
- Optimizing the xWORM assay for monitoring hookworm larvae motility.Frontiers in parasitology · 2023Article
- A self-purifying microfluidic system for identifying drugs acting against adult schistosomes.Royal Society open science · 2022Article
- Advances in diagnosis and control of anthelmintic resistant gastrointestinal helminths infecting ruminants.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Real-time and automated monitoring of antischistosomal drug activity profiles for screening of compound libraries.iScience · 2022Article
- Natural-Product-Based Solutions for Tropical Infectious Diseases.Clinical microbiology reviews · 2021Review
- Mitochondria as a Potential Target for the Development of Prophylactic and Therapeutic Drugs against Schistosoma mansoni Infection.Antimicrobial agents and chemotherapy · 2021Article
- Identification of hit compounds with anti-schistosomal activity on in vitro generated juvenile worms in cell-free medium.PLoS neglected tropical diseases · 2021Article
- Schistosomiasis Drug Discovery in the Era of Automation and Artificial Intelligence.Frontiers in immunology · 2021Review
- Parallelized Impedance-Based Platform for Continuous Dose-Response Characterization of Antischistosomal Drugs.Advanced biosystems · 2020Article
- Determining the viability of Schistosoma mansoni cercariae using fluorescence assays: An application for water treatment.PLoS neglected tropical diseases · 2020Article
- Multi-center screening of the Pathogen Box collection for schistosomiasis drug discovery.Parasites & vectors · 2019Article
- The antioxidants resveratrol and N-acetylcysteine enhance anthelmintic activity of praziquantel and artesunate against Schistosoma mansoni.Parasites & vectors · 2019Article
- Life cycle maintenance and drug-sensitivity assays for early drug discovery in Schistosoma mansoni.Nature protocols · 2019Article
- Article
- Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.Antimicrobial agents and chemotherapy · 2018Article
Corrections and comments
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5 authors.
Funding
Abstract
Infection with helminth parasites causes morbidity and mortality in billions of people and livestock worldwide. Where anthelmintic drugs are available, drug resistance is a major problem in livestock parasites, and a looming threat to public health. Monitoring the efficacy of these medicines and screening for new drugs has been hindered by the lack of objective, high-throughput approaches. Several cell monitoring technologies have been adapted for parasitic worms, including video-, fluorescence-, metabolism enzyme- and impedance-based tools that minimize the screening bottleneck. Using the xCELLigence impedance-based system we previously developed a motility-viability assay that is applicable for a range of helminth parasites. Here we have improved substantially the assay by using diverse frequency settings, and have named it the xCELLigence worm real-time motility assay (xWORM). By utilizing strictly standardized mean difference analysis we compared the xWORM output measured with 10, 25 and 50 kHz frequencies to quantify the motility of schistosome adults (human blood flukes) and hatching of schistosome eggs. Furthermore, we have described a novel application of xWORM to monitor movement of schistosome cercariae, the developmental stage that is infectious to humans. For all three stages, 25 kHz was either optimal or near-optimal for monitoring and quantifying schistosome motility. These improvements in methodology sensitivity should enhance the capacity to screen small compound libraries for new drugs both for schistosomes and other helminth pathogens at large.
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